淋巴瘤相关基因C9orf41的研究

  目的 为了探讨淋巴瘤发生的分子机制及有关的基因改变，研究淋巴瘤与反应性增生淋巴结的基因表达差异。方法 采用生物信息学方法对一条淋巴瘤高表达新基因C9orf41进行初步分析。分别将淋巴瘤淋巴结标本和反应性增生淋巴结标本的mRNA与表达谱芯片杂交，通过信号扫描、处理后获得两者的表达差异基因。并用生物信息学方法对一条新基因C9orf41进行初步分析，预测了其基因结构、染色体定位、编码蛋白质的理化性质、亚细胞定位、蛋白质功能域等信息。并对多物种中的相似性蛋白进行系统进化分析。结果 表达谱芯片发现了152条差异表达基因。对淋巴瘤相关新基因C9orf41的上述性质进行了有效的预测，基本明确了该基因编码蛋白为一线粒体蛋白。结论 新基因C9orf41可能与淋巴瘤发生有关，应作进一步的功能研究。

Study on lymphoma-associated gene C9orf41

Objective Lymphoma is considered a common primary tumor in hematological system, but the mechanism involved in its pathogenesis has not been well understood. To explore the molecular mechanism and the responsible genes for lymphoma, we performed gene expression profiling of lymphoma and reactive lymph node using complementary DNA (cDNA) microarray, and performed an initial bioinformatics analysis on a novel gene, C9orf41, which is highly expressed in lymph node of lymphoma. Methods The mRNA of the tissue from the lymphoma and reactive lymph node are marked with biotin respectively and hybridized with expression profile microarray, and the differential expressed genes are obtained. Initial bioinformatics analysis is performed on a novel gene named C9orf41 which had no functional researches at present. Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain and so on are predicted, and the systematic evolution analysis is performed on the similar proteins among several species. Results Using expression profile microarray, 152 differential expressed genes are uncovered. Efficient bioinformatics analysis have fundamentally identified that C9orf41 is a mitochondrial protein. Conclusion By bioinformatics analysis, C9orf41 may involved in the neoplastic transformation of lymphoma. It is anticipated that the new insights will help to identify promising molecular targets for therapeutic intervention