高灵敏共振瑞利散射技术快速检测药物中的美司那

目的 建立快速、准确测定药物中美司那的高灵敏共振瑞利散射（resonance Rayleigh scattering，RRS）新方法。方法 在BR弱碱性溶液中，亮绿与美司那以静电作用生成的缔合物使RRS信号显著增强，RRS强度在最大RRS峰处与美司那的浓度有线性关系。检测波长为344 nm。结果 在pH 7.75 BR缓冲溶液中，亮绿与美司那结合生成绿色二元离子缔合物，产生以294，344，468 nm为特征峰的新RRS光谱。最大RRS峰位于344 nm，线性范围为0.006~0.41 mg·L^-1，检出限为0.005 2 mg·L^-1。该法用于市售美司那药物中美司那的测定，加样回收率和相对标准偏差（RSD，n=6）分别为98.3%~103%和1.6%~2.3%。结论 该法简便、快速，有高灵敏度和高选择性，可用于实际药物中美司那的测定。

Rapid Detection of Mesna in Drugs by Highly Sensitive Resonance Rayleigh Scattering Technique

OBJECTIVE A new high sensitivity resonance Rayleigh scattering method for the rapid and accurate determination of mesna in drugs was established. METHODS In the weakly basic solution of BR, bright green combined with mesna by electrostatic interaction to form ionic association complexs, which led to a significantly enhanced of the resonance Rayleigh scattering (RLS) signal. The RRS intensity has a good linear relationship with mesna concentration at the peak of the maximum resonance Rayleigh scattering. The detection wavelength: 344 nm. RESULTS In a BR buffer solution of pH 7.75, bright green combined with mesna to form a green binary ionic association. New resonance Rayleigh scattering spectra with characteristic peaks at 294 nm, 344 nm, and 468 nm was produced. The maximum resonance Rayleigh scattering peak was located at 344 nm, linear range was 0.006~0.41 mg/L, the detection limit was 0.0052 mg/L. This method has been applied to determine the content of mesna in commercially available mesna drugs, with the spiked recoveries and relative standard deviations (RSD, n=6) were 98.3% to 103% and 1.6% to 2.3%, respectively. CONCLUSION The method was simple, rapid, not only high sensitivity, but also high selectivity. This method was applied to the determination of mesna in the actual drug, and the results satisfactory.