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| 腺病毒构建过表达睫状神经营养因子对骨髓间充质干细胞的影响 | |
| 引用本文： | 林雯,徐国兴. 腺病毒构建过表达睫状神经营养因子对骨髓间充质干细胞的影响[J]. 国际眼科杂志, 2020, 20(12): 2039-2043 |
| 作者姓名： | 林雯 徐国兴 |
| 作者单位： | 350001 中国福建省福州市,福建省立医院眼科;350005 中国福建省福州市,福建医科大学附属第一医院眼科福建省眼科研究所;350005 中国福建省福州市,福建医科大学附属第一医院眼科福建省眼科研究所 |
| 基金项目： | 国家自然科学基金资助项目(No.81770948); 福建省科技创新领军人才基金资助项目(No.2016B011) |
| 摘 要： | 目的：构建过表达睫状神经营养因子(CNTF)的腺病毒，利用腺病毒载体转染骨髓间充质干细胞，使其在体外持续高效表达CNTF，为视网膜病变的体外研究提供新途径。 方法：构建空载GFP-腺病毒及CNTF-腺病毒，取第3代BMSCs用于转染实验，分为3组：空白对照组(未转染腺病毒组)、阴性对照组(GFP-腺病毒转染组)和实验组(CNTF-腺病毒转染组)。ELISA测转染后1、2、3d各组上清液的CNTF蛋白分泌量。 结果：成功构建空载GFP-腺病毒及CNTF-腺病毒， 实验组的CNTF表达量明显高于空白对照组和阴性对照组，差异具有统计学意义(P<0.05)。 结论：利用腺病毒载体可使BMSCs在体外持续高效表达CNTF。 |
| 关 键 词： | 骨髓间充质干细胞 睫状神经营养因子 视网膜病变 腺病毒 |
| 收稿时间： | 2020-05-24 |
| 修稿时间： | 2020-11-11 |
Effects of ciliary neurotrophic factor over-expression by adenovirus on bone marrow mesenchymal stem cells |
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| Wen Lin and Guo-Xing Xu. Effects of ciliary neurotrophic factor over-expression by adenovirus on bone marrow mesenchymal stem cells[J]. International Eye Science, 2020, 20(12): 2039-2043 | |
| Authors: | Wen Lin and Guo-Xing Xu |
| Affiliation: | Department of Ophthalmology, Fujian Provincial Hospital, Fuzhou 350001, Fujian Province, China; Fujian Institute of Ophthalmology and Fujian Institute of Ophthalmology; Department of Ophthalmology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China |
| Abstract: | AIM: To establish the model of bone marrow mesenchymal stem cells(BMSCs)over-expressing ciliary neurotrophic factor(CNTF)by applying adenovirus. It may provide novel strategies for in-vitro investigations of retinopathies. METHODS: GFP-adenovirus and CNTF-adenovirus were synthesized then used to transfect BMSCs of passage 3. Blank control group(without adenovirus transfected group), negative control group(GFP-adenovirus transfected group), and experimental group(CNTF-adenovirus transfected group)were included in this study. On the 1, 2, 3d post-transfected, ELISA assay was applied to examine CNTF protein-secretion in the supernate. RESULTS: GFP-adenovirus and CNTF-adenovirus models were successfully established. The CNTF protein levels in the supernate were higher in experimental group than those in the blank control group and negative control group(P<0.05). CONCLUSION: CNTF-modified BMSCs by adenovirus could efficiently secrete CNTF protein in-vitro. |
| Keywords: | bone marrow mesenchymal stem cells ciliary neurotrophic factor retinopathis adenovirus |
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