苦豆碱对缺氧/复氧诱导人脐静脉内皮细胞凋亡的影响

目的：探讨苦豆碱对缺氧/复氧诱导的人脐静脉内皮细胞凋亡的影响。方法：培养人脐静脉内皮细胞，随机分为对照组、缺氧/复氧组、苦豆碱（20、50和100 mg/L）预处理组和苦豆碱预处理+LY294002组，并进行相应处理。MTT法检测细胞增殖，流式细胞术检测细胞凋亡，比色法检测Caspase?3活性，Western blot法检测Bax、Bcl?2、Akt和p?Akt蛋白表达。结果：苦豆碱可提高缺氧/复氧抑制的人脐静脉内皮细胞增殖（P < 0.05），显著减少缺氧/复氧诱导的细胞凋亡（P < 0.05），逆转缺氧/复氧引起的Caspase?3活性和Bax表达的增加、Bcl?2表达的降低，提高Bcl?2/Bax值（P < 0.05）。此外，苦豆碱预处理可明显上调缺氧/复氧降低的p?Akt蛋白表达（P < 0.05），而PI3K/Akt抑制剂LY294002明显逆转苦豆碱抗缺氧/复氧诱导的凋亡作用（P < 0.05）。结论：苦豆碱通过激活PI3K/Akt通路抑制缺氧/复氧诱导的人脐静脉内皮细胞凋亡。

Effects of aloperin on the apoptosis of human umbilical vein endothelial cells induced by hypoxia/reoxygenation

Objective: To investigate the protective effect of aloperin on the apoptosis of human umbilical vein endothelial cells (HUVEC) induced by hypoxia/reoxygenation. Methods: HUVEC cells were cultured and randomly divided into control group, hypoxia/reoxygenation group, aloperin (20, 50 and 100 mg/L) pretreatment group and aloperin+LY294002 group, and treated accordingly. Cell proliferation and apoptosis was detected by MTT assay and flow cytometry, respectively. Caspase-3 activity was detected by colorimetry, and protein expression of Bax, Bcl-2, Akt and p-Akt were detected by Western blot. Results: Aloperin inhibited the decrease of proliferation and increase apoptosis by hypoxia/reoxygenation in human umbilical vein endothelial cells (P<0.05). Aloperin decreased the increase of Caspase-3 activity and Bax expression induced by hypoxia/reoxygenation, while aloperin increased the downregulation of Bcl-2 induced by hypoxia/reoxygenation, and increased Bcl-2/Bax value (P<0.05). In addition, aloperin significantly upregulated p-Akt protein expression (P<0.05), while the anti-apoptosis effect of aloperin against hypoxia/reoxygenation was significantly reversed by PI3K/Akt inhibitor LY294002 (P<0.05). Conclusion: Aloperin could inhibit hypoxia/reoxygenation-induced apoptosis of HUVEC cells by activating the PI3K/Akt pathway.