Box-Behnken响应面法优化高良姜挥发油的包合工艺研究

目的 优化胶体磨法制备高良姜挥发油β-环糊精(β-CD)包合物的工艺。方法 在单因素试验基础上采用Box-Behnken响应面法,以包合物得率和包合率为评价指标,考察β-CD与高良姜挥发油的比例、水与β-CD的比例以及包合时间对包合工艺的影响；通过红外光谱法和显微影像法初步验证高良姜挥发油-β-CD包合物的生成。结果 高良姜挥发油的最佳包合工艺为β-CD与高良姜挥发油的比例为10：1,水与β-CD的比例为5：1,包合时间为45min。通过红外光谱法和显微影像法初步证明了高良姜挥发油包合物的生成。结论 胶体磨法包合高良姜挥发油合理、可行。

Optimization for Inclusion Process of Alpinia Officinarum Hance Volatile Oil by Box-Behnken Response Surface Methodology

OBJECTIVE To optimize the preparation process of Alpinia officinarum Hance volatile oil wrapped with b-cyclodextrin(b-CD) using the colloid mill method. METHODS With yield of inclusion compound and inclusion rate as evaluation indexes, the single factor test combining Box-Behnken response surface methodology was used to study the effects of the ratio of b-CD to Alpinia officinarum Hance volatile oil, the ratio of water to b-CD, and inclusion time on the inclusion process. Inclusion complexes were verified by IR and microscopic image. RESULTS The optimized parameters of inclusion were as follows:the ratio of b-CD to Alpinia officinarum Hance volatile oil was 10:1, the ratio of water to b-CD was 5:1, and the inclusion time was 45 min. CONCLUSION The inclusion of Alpinia officinarum Hance volatile oil by colloid mill is reasonable and feasible.