B7-H3对人肝癌细胞生物学特性的影响及临床意义

目的 研究下调B7-H3分子的表达对人肝癌(HCC)细胞生物学特性的影响,探讨B7-H3表达对HCC组织发生发展的临床意义。 方法 选择两株B7-H3表达水平较高的HCC细胞,通过RNA干扰技术下调HCC细胞B7-H3的表达,采用CCK-8、流式细胞术、细胞划痕和Transwell小室等技术,观察HCC细胞在增殖、凋亡、迁移及侵袭等方面的改变; 制备不同复发时间(1年内复发和超过1年复发)的HCC组织芯片,采用免疫组织化学法检测B7-H3在80例HCC组织及癌旁肝组织中的表达,进一步分析B7-H3的表达与HCC复发转移及其他临床病理参数的关系。 结果 选择B7-H3表达水平较高的HCC细胞株SK-hep-1和MHCC97L,转染B7-H3 siRNA后构建B7-H3下调的细胞模型,发现下调B7-H3蛋白后,HCC细胞的侵袭和迁移能力显著下降(P<0.05),而增殖和凋亡能力未见明显影响; B7-H3在肝癌组织中的阳性率(73.33%)高于癌旁肝组织(54.67%)(P=0.041); B7-H3阳性表达率与HCC分化程度(P=0.012)、复发时间(P=0.001)、转移情况(P=0.007)呈正相关,而与年龄、性别、肿块大小、甲胎蛋白(AFP)等临床病理特征无明显相关。 结论 B7-H3可作为HCC复发与转移的潜在指标,下调B7-H3表达可为抑制HCC侵袭提供新思路。

Effect of B7-H3 on Biological Characteristics of Human Hepatoma Cells and its Clinical Significance

Objective To investigate the effects of down-regulation of B7-H3 on the biological characteristics of human hepatocellular carcinoma(HCC)cells, and to explore the clinical significance of down-regulation of B7-H3 on the development of HCC. Methods The proliferation, apoptosis, migration and invasion of HCC cells were observed by cell scratch and Transwell chamber techniques. The expression of B7-H3 in 80 HCC tissues and adjacent liver tissues was detected by immunohistochemical method, and the relationship between B7-H3 expression and clinicopathological parameters of HCC was analyzed. Results B7-H3 down-regulated HCC cell model was constructed by choosing SK-hep-1 and MHCC97L with high expression of B7-H3 and transfection of B7-H3 siRNA. The positive rate of B7-H3 in HCC tissues was 73.33%, which was significantly different from that in adjacent liver tissues(54.67%)(P=0.041). The positive rate of B7-H3 was positively correlated with the differentiation degree of HCC(P=0.012), recurrence time(P=0.001), and metastasis(P=0.007), but positively correlated with age, sex, and tumor size. Conclusions B7-H3 can be used as a potential indicator of recurrence and metastasis of HCC. Downregulation of B7-H3 expression may provide a new way to inhibit the invasion of HCC.