复合碳酸钙空腔纳米粒的制备及性能评价

目的 制备甘草次酸/海藻酸钠修饰碳酸钙空腔纳米粒并进行体外评价。方法 以可溶性淀粉为模板剂制备中空球状碳酸钙纳米粒（CaCO3 Nps）；在非均相体系中合成了甘草次酸/海藻酸钠聚合物（GA-ALG）；并以聚合物（GA-ALG）为壳以中空结构的碳酸钙纳米粒为核，合成了壳核结构的GA-ALG-CaCO3 Nps。采用Malvern粒度分析仪测定纳米粒子的粒度分布和Zeta电位，并通过SEM对纳米粒的形态进行表征。应用荧光分光光度计评价载盐酸阿霉素（DOX）纳米粒的载药量、包封率及体外释放特征。结果 纳米粒分布均一，平均粒径为（425.4±31.1）nm，PDI为0.289，Zeta 电位为（-17.0±0.3）mV。药物的载药量为（13.06±0.51）%，包封率为（78.35±3.08）%。；体外释放结果显示，纳米粒具有一定的缓释作用。结论 GA-ALG-CaCO3 Nps作为新型的药物载体，具有良好的pH响应性，并能显著提高载药量，还具有明显的缓释效果，为新型的纳米给药系统的深入研究提供参考。

Preparation and Properties Evaluation of Composite Calcium Carbonate Hollow Nanoparticles

OBJECTIVE To prepare glycyrrhetinic acid/sodium alginate modified calcium carbonate nanoparticles and to evaluate its release properties in vitro. METHODS The hollow spherical calcium carbonate nanoparticles (CaCO3 Nps) were prepared by using Soluble starch as organic templates. Glycyrrhetinic acid / sodium alginate polymer (GA-ALG) was synthesized in heterogeneous system. GA-ALG-CaCO3 Nps with shell-core structure were synthesized by using GA-ALG as shell and hollow CaCO3 nanoparticles as core. The particle size distribution and zeta potential of nanoparticles were measured by Malvern particle size analyzer, and the morphology of nanoparticles was characterized by SEM. The drug loading, entrapment efficiency and in vitro release characteristics of doxorubicin hydrochloride (DOX) nanoparticles was evaluated by fluorescence spectrophotometer. RESULTS The average size of nanoparticles was (425.4±31.1)nm,PDI was 0.289, zeta potential was (-17.0±0.3)mV, drug loading was (13.06 ± 0.51)%,encapsulation efficiency was (78.35 ± 3.08)%. In vitro release results showed that nanoparticles had a certain sustained release effect. CONCLUSION As a new drug carrier, GA-ALG-CaCO3 Nps has good pH responsiveness, can significantly increase drug loading, and has obvious sustained release effect. It also has obvious sustained release effect, which provides a reference for the further study of new nano-drug delivery system.