青藤碱通过调控NF-kB信号通路抑制胰腺癌Capan-1细胞增殖并诱导细胞凋亡

目的 探讨青藤碱对胰腺癌Capan-1细胞增殖、凋亡的影响及机制。方法 CCK8法检测细胞活力，DAPI/TUNEL双染检测细胞凋亡，流式细胞术Annexin V-FITC/PI染色法检测细胞凋亡率，Western blotting检测cleaved caspase-3蛋白、细胞核NF-κB蛋白表达。结果 CCK8结果表明青藤碱抑制胰腺癌Capan-1细胞增殖，并具有时间、浓度依赖性；DAPI/TUNEL染色结果表明，在青藤碱作用下，胰腺癌Capan-1细胞凋亡增多；流式细胞术Annexin V-FITC/PI结果表明，在青藤碱作用下，胰腺癌Capan-1细胞凋亡率增高；Western blotting结果表明，在青藤碱作用下，胰腺癌Capan-1细胞cleaved caspase-3表达升高，细胞核NF-kB表达降低。TNF-α降低青藤碱对胰腺癌Capan-1细胞增殖抑制作用、凋亡诱导作用和cleaved caspase-3表达的上调作用。结论 青藤碱通过调控NF-kB信号通路抑制胰腺癌Capan-1细胞增殖并诱导细胞凋亡。

Sinomenine Inhibits Proliferation and Induces Apoptosis of Pancreatic Cancer Capan-1 Cells by Regulating NF-kB Signaling Pathway

OBJECTIVE To investigate the effect and mechanism of sinomenine on apoptosis of pancreatic cancer Capan-1 cells. METHODS CCK8 assay was used to detect cell viability. DAPI/TUNEL staining was used to detect cell apoptosis. Flow cytometry Annexin V-FITC/PI was used to detect cell apoptotic rate. Western blotting was used to detect the expression of cleaved caspase-3 and nuclear NF-kB protein. RESULTS Result of CCK8 assay showed that sinomenine can inhibit the proliferation of Capan-1 cells effectively in a dose and time dependent manner. Result of DAPI/TUNEL staining showed that the apoptosis of Capan-1 cells increased by treated with sinomenine. Flow cytometric analysis staining with Annexin V-FITC/PI showed that the apoptosis rates of Capan-1 cells increased by treated with sinomenine. Result of Western blotting analysis showed that the expression of cleaved caspase-3 increaed and the expression of nuclear NF-kB decreased in Capan-1 cells by treated with sinomenine. TNF-a could reversed the effect of sinomenine on the proliferation and apoptosis of Capan-1 cell. TNF-a could reversed the up-regulated effect of sinomenine on the expression of cleaved caspase-3 in Capan-1 cells. CONCLUSION Sinomenine inhibits proliferation and induces apoptosis of pancreatic cancer Capan-1 cells by regulating the NF-kB signaling pathway.