牙周非手术治疗对重度慢性牙周炎患者龈沟液中MMP-8和TIMP-1水平的影响

目的 评价牙周非手术治疗对重度慢性牙周炎患牙龈沟液(GCF)中基质金属蛋白酶-8(MMP-8)及其组织抑制剂-1(TIMP-1)水平的影响。 方法 选取20颗重度牙周炎患牙,牙周非手术治疗前后检查菌斑指数(PLI)、牙周袋深度(PD)、出血指数(BI)、附着丧失(AL),收集GCF,采用酶联免疫吸附法(ELISA)检测GCF中MMP-8和TIMP-1的含量。 结果 牙周非手术治疗能显著改善PLI、PD、BI和GCF的量(P<0.01)以及AL(P<0.05)。GCF中MMP-8和TIMP-1的水平在治疗前后无明显变化; 除TIMP-1与治疗后PLI呈正相关关系外(P<0.05),MMP-8和TIMP-1与其他临床指标均无显著相关性。 结论 牙周非手术治疗能有效改善重度慢性牙周炎的临床指标,但不能改变GCF中MMP-8和TIMP-1的水平。

Nonsurgical Treatment on MMP-8 and TIMP-1 Levels in GingivalCrevicular Fluid from Advanced Chronic Periodontitis Patients

Objective The study of the influence of nonsurgical treatment was determined on amounts of matrix metalloproteinases-8(MMP-8)and tissue inhibitiors of metalloproteinases-1(TIMP-1)in gingival crevicular fluid(GCF)from teeth with advanced chronic periodontitis. Methord The study consisted of 20 teeth with advanced chronic periodontitis from 7 chronic periodontitis patients. The amounts of MMP-8 and TIMP-1 in GCF were assayed by ELISA. Patients were treated by nonsurgical treatment and oral hygiene instructions. GCF MMP-8 collection and clinical measurements were done before and 4week after nonsurgical treatment. Results The amount of different clinical indices with respect to the nonsurgical treatment were apparent: PLI, PD, BI, and GCF showed P<0.01 whereas AL showed P<0.05. However, no significant difference was observed on the level of MMP-8 and TIMP-1 with respect to the nonsurgical treatment. No positive correlation was found between the clinical indices and the level of MMP-8 and TIMP-1 except the level of TIMP-1 and the amount of PLI after the nonsurgical treatment(P<0.05). Conclusions Advanced chronic periodontitis showed improvement on the clinical indices after the nonsurgical treatment. No significant difference was found on the level of MMP-8 and TIMP-1 in GCF before and after the nonsurgical treatment.