EGF与bFGF对体外大鼠巩膜成纤维细胞增殖的影响

目的研究表皮生长因子（EGF）和碱性成纤维生长因子（bFGF）在体外对大鼠巩膜成纤维细胞生长的影响。为进一步了解近视发生的分子机制提供依据。方法采用植块法培养大鼠巩膜成纤维细胞。细胞经传代纯化鉴定后，取第3～4代细胞加入不同浓度的EGF（0．1～200ng／mL）、bFGF（0．1～50ng／mL）。分别培养24、48、72h用四甲基偶氮唑盐（MTT）法检测细胞的增殖密度。观察不同浓度、时间下细胞增殖情况。结果发现0．1～100ng／mL的EGF和0．1～50ng／mL的bFGF对大鼠巩膜成纤维细胞均有明显的促增殖作用，且呈剂量相关性。结论一定浓度的EGF、bFGF对体外培养的大鼠巩膜成纤维细胞的生长具有促进作用。

Effect of EGF and bFGF on scleral fibroblasts proliferation in vitro

Purpose To study the effects of epidermal growth factor(EGF) and basic fibroblast growth factor(bFGF) on scleral fibroblasts proliferation in vitro. Methods The rat scleral fibroblasts were grown in tissue culture,then were treated with different doses of EGF(0.1,1.0,10,100 and 200 ng/mL) or bFGF(0.1,0.5,1.0,10 and 50 ng/mL) individually.The effects of EGF and bFGF on scleral fibroblasts proliferation were measured by MTT assay. Results EGF induced proliferation significantly at concentrations of 0.1,1.0,10 and 100 ng/mL in comparison with control,but in 200 ng/mL EGF didn't induced proliferation.bFGF induced proliferation significantly at concentrations of 0.1,0.5,(1.0,)10 and 50 ng/mL. Conclusions The results suggested that EGF and bFGF could regulate the growth of rat scleral fibroblasts in vitro.