人参皂甙Rg3抑制小鼠肝癌淋巴道转移作用及其对免疫功能的影响

目的：观察人参皂甙Rg3抑制小鼠肿瘤生长及抗淋巴结转移的作用及对免疫功能的影响。方法：以Hca-F25//6A3-F（F）接种于615近交系小鼠，建立肝癌淋巴道转移动物模型，分为5组：Rg3预防组（接种肿瘤前给Rg3）、Rg3治疗组（Rg3）、阳性对照组（PDD）、联合治疗组（Rg3＋PDD）和阴性对照组（生理盐水），比较各组的原发瘤抑制率和转移淋巴结抑瘤率，并通过流式细胞仪方法分析各组免疫指标CD4／CD8的变化。结果：联合治疗组原发瘤的抑瘤率明显提高，与阴性对照组相比具有统计学上明显差异（P〈0．05）；淋巴结转移抑制率含Rg3组均较阴性对照组高；Rg3预防组和Rg3治疗组CD4／CD8比值与阴性对照组相比升高，具有统计学上明显差异（P〈0．05）。结论：人参皂甙Rg3具有明显的抗肿瘤作用，可以增强化疗药物PDD的抗癌作用，及抑制淋巴道转移作用，并提高荷瘤小鼠免疫功能。

Inhibition of the lymphatic metastasis and influence on immune functions in mice treated by ginsenoside Rg3

Objective:To observe the inhibition of lymphatic metastasis of tumor and influence on immune functions in tumor mice induced by ginsenoside Rg3 treatment.Methods:The transplanted tumor lymphatic metastasis model was established with Hca-F_(25)//6A_3-F(F) in 615 inbred mice,and the tumor mice were divided into five groups:treated with ginsenoside Rg3 ahead,ginsenoside Rg3,povitive group(cisplatin),ginsenoside Rg3+cisplatin,and control group(saline),observing inhibition ratio of the primary tumor and metastatic lymph node,and CD_4/CD_8 ratio.Results:The inhibition ratio of the primary tumor was significantly higher in the ginsenoside Rg3+cisplatin group;the inhibition ratio of metastatic lymph node was higher in ginsenoside Rg3-containing group,comparing with the control group.And CD_4/CD_8 was increasing in the ginsenoside Rg3 containing group.Conclusion:Ginsenoside Rg3 play a role in anti-lymphatic metastasis,enhancing anti-tumor effect of cytotoxic drug,modulating the immune system.