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| 多药耐药铜绿假单胞菌氨基糖苷类耐药相关基因研究 | |
| 引用本文： | 崔进,赵水娣,秦湧,高天明,王新云,冯旰珠.多药耐药铜绿假单胞菌氨基糖苷类耐药相关基因研究[J].中华医院感染学杂志,2010,20(20). |
| 作者姓名： | 崔进 赵水娣 秦湧 高天明 王新云 冯旰珠 |
| 作者单位： | 1. 南京医科大学第二附属医院呼吸科,江苏,南京,210011 2. 南京医科大学第二附属医院细菌室,江苏,南京,210011 3. 南京医科大学第二附属医院感染性疾病科,江苏,南京,210011 |
| 基金项目： | 江苏省卫生厅资助项目 |
| 摘 要： | 目的研究临床分离的多药耐药铜绿假单胞菌(MDR-PEA)常见氨基糖苷类修饰酶及甲基化酶基因的存在状况。方法收集2009年2月-2010年4月从医院住院患者分离出来的MDR-PAE20株,采用聚合酶链反应(PCR)及序列分析的方法分析对6种甲基化酶及8种氨基糖苷类修饰酶基因进行检测及测序。结果 20株MDR-PAE中,甲基化酶基因rmtB阳性率为15.0%;氨基糖苷类修饰酶基因aac(3)-Ⅱ、aac(6′)-Ⅰb、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ的阳性率分别为40.0%、10.0%、20.0%、15.0%、10.0%,未检出aac(3)-Ⅰ、ant(4′)-Ⅰ及aph(3′)-Ⅱb。结论产氨基糖苷类修饰酶是MDR-PAE对氨基糖苷类药物耐药的主要机制,非产氨基糖苷类修饰酶MDR-PAE株对氨基糖苷类药物耐药可能与其他机制有关。 |
| 关 键 词： | 铜绿假单胞菌 多药耐药 氨基糖苷类修饰酶 甲基化酶基因 |
Investigation of Related Genes of Aminoglycoside Resistant on Multi-drug Resistance Pseudomonas aeruginosa |
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| CUI Jin,ZHAO Shui-di,QIN Yong,GAO Tin-ming,WANG Xin-yun,FENG Gan-zhu.Investigation of Related Genes of Aminoglycoside Resistant on Multi-drug Resistance Pseudomonas aeruginosa[J].Chinese Journal of Nosocomiology,2010,20(20). | |
| Authors: | CUI Jin ZHAO Shui-di QIN Yong GAO Tin-ming WANG Xin-yun FENG Gan-zhu |
| Institution: | CUI Jin,ZHAO Shui-di,QIN Yong,GAO Tin-ming,WANG Xin-yun,FENG Gan-zhu(The Second Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210011,China) |
| Abstract: | OBJECTIVE To investigate the distribution of methylase genes and aminoglycosides modification enzyme in multi-drug resistant Pseudomonas aeruginosa(MDR-PAE)strains isolated from clinics.METHODS A total of 20 multi-drug resistant P.aeruginosa(MDR-PEA)strains isolated from Feb 2009 to Apr 2010 were collected,6 kinds of methylase and 8 kinds of aminoglycosides modification enzyme genes were analyzed by PCR and DNA sequencing.RESULTS Among the 20 strains of MDR-PEA,the positive rate of methylase carrying rmtB w... |
| Keywords: | Pseudomonas aeruginosa Multi-drug resistance Aminoglycosides modification enzyme Methylase genes |
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