|
|||||
|
|
| ELISA IFAT和PCR/生物素探针检测中枢神经系统疾病中弓形虫感染 | |
| 引用本文： | 张洪花,王昌源,汪照国,韩广东,魏庆宽,李玉娥. ELISA IFAT和PCR/生物素探针检测中枢神经系统疾病中弓形虫感染[J]. 中国血吸虫病防治杂志, 2002, 14(6): 450-452 |
| 作者姓名： | 张洪花 王昌源 汪照国 韩广东 魏庆宽 李玉娥 |
| 作者单位： | 1. 山东省青岛市疾病控制中心,青岛,266033 2. 山东省寄生虫病防治研究所 3. 山东省济宁市精神病防治院 |
| 摘 要： | 目的 比较 ELISA、IFAT和 PCR/生物素探针检测弓形虫感染的效果 ,了解中枢神经系统疾病中弓形虫感染的情况。方法 应用 EL ISA、IFAT和 PCR/生物素探针 (PCR-BP)对 164例脑囊虫病、10 8例疑似脑囊虫病、88例原发性癫痫患者和 115例健康人群进行了血清弓形虫抗体及血液中弓形虫特异序列 DNA检测和比较。结果 ELISA和 IFAT检测各组人群抗弓形虫抗体阳性率分别为 15 .85 %、14 .81%、13 .64 %和 6.0 9% ,14 .0 2 %、14 .81%、14 .77%和 6.0 9%。PCR-BP检测各组弓形虫特异性 DNA阳性率分别为 2 .44 % (4 /164 )、3 .70 % (4 /10 8)、3 .41% (3 /88)、0 (0 /115 )。结论 PCR/生物素探针适合诊断现症弓形虫感染 ,而 EL ISA和 IFAT可作为弓形虫感染的筛选方法。脑囊虫病、疑似脑囊虫病和原发性癫痫患者中存在弓形虫感染 |
| 关 键 词： | 弓形虫 DNA 抗体 PCR 脑囊虫病 原发性癫痫 |
| 文章编号： | 1005-6661(2002)06-0450-03 |
| 修稿时间： | 2002-08-28 |
DETECTION OF TOXOPLASMA GONDII INFECTION IN CENTRAL NERVOUS SYSTEM DISEASES WITH ELISA IFAT AND PCR/BP |
|
| Zhang Honghua ,Wang Changyuan ,Wang Zhaoguo ,Han Guandong ,Wei Qingkuan ,Li Yue Qingdao Center of Diseases Contro,Qingdao ,China, Shandong Institute of Parasitic Diseases,Jining,China, Jining Psychiatric Hospital,Shandong,China. DETECTION OF TOXOPLASMA GONDII INFECTION IN CENTRAL NERVOUS SYSTEM DISEASES WITH ELISA IFAT AND PCR/BP[J]. Chinese journal of schistosomiasis control, 2002, 14(6): 450-452 | |
| Authors: | Zhang Honghua Wang Changyuan Wang Zhaoguo Han Guandong Wei Qingkuan Li Yue Qingdao Center of Diseases Contro Qingdao China Shandong Institute of Parasitic Diseases Jining China Jining Psychiatric Hospital Shandong China |
| Affiliation: | Zhang Honghua 1,Wang Changyuan 2,Wang Zhaoguo 1,Han Guandong 2,Wei Qingkuan 2,Li Yue 3 1 Qingdao Center of Diseases Contro1,Qingdao 266033,China, 2 Shandong Institute of Parasitic Diseases,Jining,China, 3 Jining Psychiatric Hospital,Shandong,China |
| Abstract: | Objectives To compare the effectiveness of detecting Toxoplasma gondii infection with ELISA, IFAT and PCR/Biotin probe(PCR/BP), and find out the situation of toxoplasmatic infection in central nervous system diseases.Methods Using ELISA, IFAT and PCR/BP, the specific antibodies and DNA sequence of Toxoplasma gondii were detected in the sera of 164 neurocysticercosis cases,108 cases with suspected neurocysticercosis, 88 primary epilepsy cases and 115 health people. Results The antibody positive rates of the four groups above metioned by ELISA and IFAT were 15 85%, 14 81%,13 64%, and 6 09%; 14 02%,14 81%,14 77%, and 6 09%, respectively. The specific DNA positive rates were 2 44%,(4/164), 3 70%(4/108),3 41%,(3/88),0(0/115) respectively. Conclusion PCR/BP is sutitable for detection of active toxoplasmatic infection, while ELISA and IFAT are useful in screening examination.Toxoplasmiatic infections existed in cases of clinically diagnosed nuerucysticercosis, suspected neurocysticercosis and primary epilepsy |
| Keywords: | Toxoplasma gondii DNA Antibody PCR Neurocysticercosis Primary epilepsy |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |