HLA antigens expressed on human ervthroid burst forming cells |
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| Authors: | Yokiko Ohe Hiroshi Hara Kiyovasu Nagai |
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| Institution: | Department of International Medicine, Hyogo college of Medicine, Nishinomiya, Hyogo, Japan;Division of Blood Transfusion, Hyogo college of Medicine, Nishinomiya, Hyogo, Japan |
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| Abstract: | The human major histocompatibility antigens HLA, play an important role in transplantation. To ascertain the expression of the antigens of A and B loci on the surface of human hemopoietic progenitors, the cytotoxic effect of specific anti-HLA sera was examined. Anti-HLA A2 and B5 sera were used in the present experiments. Circulating blood mononuclear cells and nucleated marrow cells, pre-treated with appropriate specific anti-HLA sera and complement, formed fewer colonies from BFU-e and CFU-c in a culture medium than the controls which were treated with autologous serum. In our experiments, the HLA antisera also killed macrophages, monocytes and T cells which carry the surface antigen. Then, the influence of macrophages, monocytes, and T cells on colony formation from BFU-e and CFU-c was examined. These results indicated that BFU-e as well as CFU-c expressed HLA antigens on their surface. |
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