异种管状皮质骨复合人重组骨形态发生蛋白与碱性成纤维细胞生长因子

背景：传统的松质骨移植虽然广泛应用于临床，但用于大段骨缺损还存在一定的局限性。目的：通过对复合人重组骨形态发生蛋白/碱性成纤维细胞生长因子(recombinant human bone morphogenetic protein-2/ basic fibroblast growth factor， rhBMP-2/bFGF)大段异种皮质骨成骨作用观察，探讨异种皮质骨修复大段骨缺损的可行性。设计、时间及地点：随机对照动物实验，于2007-01/2008-01在解放军成都军区昆明总医院动物实验室完成。材料：版纳近交系小耳猪骨，经过钻孔、酶处理等方法处理后，再在真空、冻干、吸附基础上复合rhBMP-2/bFGF，成为具有生物活性的复合皮质管状骨。经复合后每根复合骨含40 U bFGF+10 mg聚乙烯吡咯酮+0.5 mg rhBMP-2。方法：45只新西兰兔随机分成3组，于兔左桡骨中段制成2 cm的骨-骨膜缺损模型，实验组植入复合皮质管状骨、对照组植入单纯管状皮质骨、空白对照组不植入任何材料。主要观察指标：分别于术后4，8，12周各时间点取材，通过X射线检查、组织学观察等指标观察骨缺损修复情况。结果：实验组术后4周皮质骨活化，术后8周移植皮质骨两端结合处愈合，术后12周骨缺损修复较满意；对照组修复缓慢；空白对照组骨缺损未见修复。结论：近交系管状猪皮质骨复合rhBMP-2/bFGF具有良好的生物学活性，修复大段骨缺损效果显著。

Cortical xenograft combined with recombinant human bone morphogenetic protein/basic fibroblast growth factor for repairing large bone defects

BACKGROUND: Although traditional cancellated bone transplantation has been widely applied, it is still limited for the treatment of large bone defects.OBJECTIVE: To observe the osteogenesis of the composite of recombinant human bone morphogenetic protein 2 (rhBMP-2) and basic fibroblast growth factor (bFGF) in the healing process of large cortical xenograft, and to discuss the feasibility in repairing of the large bone defects.DESIGN, TIME AND SETTING: Randomized control animal experiment was performed in the animal laboratory of Kunming General Hospital of Chengdu Military Area Command of Chinese PLA between January 2007 and January 2008.MATERIALS: China Banna inbred-lined small-ear pig bone was combined with rhBMP-2/bFGF following a series of procedures such as drilling, enzyme treatment, vacuum drying, freeze drying and adsorption. The bioactive cortical xenograft composite was harvested, each bone contained 40 U bFGF + 10 mg polyvidone + 0.5 mg rhBMP-2.METHODS: Forty-five healthy New Zealand rabbits were divided into three groups randomly, and a 2-cm bone-periosteum defects were created on left radius. Experiment group was implanted with the composite cortical xenograft, control group with single cortical xenograft, and blank control group with nothing. MAIN OUTCOME MEASURES: The bone defects were examined radiographically and histologically at 4, 8, 12 weeks post-operation, separately.RESULTS: Cortical bone was activated at 4 weeks, osteosynthesis site was observed to heal at 8 weeks and the repairing result was good at 12 weeks after surgery in the experiment group; the repair was slowly in the control group; no repair was found in the blank control group.CONCLUSION: The Banna inbred-lined small-ear pig bone in combination with rhBMP-2/bFGF has an active biological characteristics, they are of significance in treatment of large bone defects.