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Protection of ferrets against influenza challenge with a DNA vaccine to the haemagglutinin |
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| Authors: | Robert G. Webster Ellen F. Fynan Joseph C. Santoro Harriet Robinson |
| Affiliation: | * Department of Virology and Molecular Biology, St Jude Children's Research Hospital, 332 North Lauderdale, PO Box 318, Memphis, TN 38101-0318, USA. † Department of Pathology, University of Tennessee, 800 N. Madison, Memphis, TN 38163, USA. ‡ Department of Natural Sciences, Worcester State, 416 Chandler Street, Worcester, MA 01602-2597, USA. ∞ Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. |
| Abstract: | Immunization of ferrets with a plasmid DNA expressing influenza virus haemagglutinin (pCMV/H1 DNA) provided complete protection from challenge with the homologous A/PR/8/34 (HINI) influenza virus. Delivery of DNA-coated gold beads by gene gun to the epidermis was much more efficient than intramuscular delivery of DNA in aqueous solution. The antibody response induced by DNA delivered by gene gun was more cross-reactive than DNA delivered in aqueous solution or after natural infection. This novel approach to vaccination against influenza may afford broader protection against antigenic drift than that provided by natural infection. |
| Keywords: | DNA vaccine influenza ferrets |
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