PTEN基因编码产物在胃癌中的表达及意义

目的观察抑癌基因PTEN编码产物在胃癌中的表达,探讨PTEN表达与胃癌发生发展、临床病理特征之间的关系.方法采用SP免疫组化方法检测PTEN在60例胃癌组织、17例异型增生和15例正常胃粘膜中的表达.结果 PTEN蛋白在正常胃粘膜、异型增生和胃癌中的阳性表达率分别为100%、76.47%、46.67%,表达差别均有显著性(P＜0.05);早期胃癌与异型增生组相比阳性率无明显差异性(P＞0.05);进展期胃癌PTEN表达显著低于早期胃癌(36.59%,68.75%,P＜0.05);且随着淋巴转移、分化程度降低、浸润深度加深、器官转移、临床分期,PTEN表达阳性率逐渐降低(P＜0.05).结论 PTEN基因编码产物在胃癌发生发展中进行性下调,PTEN蛋白表达可作为判断胃癌病理生物学行为的客观指标.

Expression of PTEN enconding product in gastric carcinoma and its significance

Objective To illustrate the significance of expression of phosphatase and tensin homolog deleted on chromosome ten(PTEN) enconding product in carcinoma of the stomach,and to evaluate its relationship with the occurrence and clinicopathological features of the disease.Methods Formalin-fixed and paraffin-embedded tissues from 60 cases of gastric carcinoma,17 cases of gastric dysplasia and 15 cases normal gastric mucosa were evaluated for the expression of PTEN by SP immunohistochemistry.Results The positive rates of PTEN protein were 100%,70.59% and 46.67% in normal gastric mucosa,dysplasia and gastric carcinoma,the difference of PTEN gene expression was significant(P<0.05);however the difference of PTEN gene expression was't significant between gastric dysplasia and early stage gastric carcinoma;PTEN was less expressed in advanced gastric dysplasia and early stage gastric carcinoma;PTEN was less expressed in advanced gastric carcinoma than in early stage gastric carcinoma;PTEN was less expressed in advanced gastric carcinoma than in early ones(36.59%,68.42%,P<0.05);The positive rates of PTEN protein decreased with lymph node metastasis,poor differentiation,deep invasion,apparatus metastasis and clinical staging(P<0.05).Conclusion PTEN may be an objective marker for pathologically biologically behavior of gastric carcinoma.