输精管结扎术后抗精子抗体在非射出精子上的存在

４８例正常育龄男子和２４例要求作输精管吻合术者，用免疫珠结合试验（ＩＢＴ）和精子－宫颈粘液接触试验（ＳＣＭＣ）分别检测其近睾端输精管液中的精子（非射出的精子）表面抗精子抗体的情况。结果显示：正常育龄男子非射出的精子上没有抗体存在；而输精管结扎术后非射出的精子上有抗精子抗体存在（ＩｇＧ：７９．４％，ＩｇＡ：３８．２％，ＳＣＭＣ：３５．５％）。这些抗体在输精管结扎术后不到１年就可发现，并保持到结扎术后３年以上。吻合术后１～３个月内，其精液中射出的精子上仍发现抗体存在。结果还提示：输精管结扎术后，不能以非射出的精子上抗体的水平去预测吻合术后射出的精子上抗体的水平；吻合术前血清抗体水平与非射出的精子上抗体水平并不一致。

Antisperm Antibodies Present on Spermatozoa before Ejaculation from Vasectomized Men

The antisperm antibodies(ASABs) coated on spermatozoa of the proximal vas deferens(sperm before ejaculation) from 48 fertile men and 24 vasectomized men(asking for vasovasostomy)were determined by Immunobead Test(IBT) and sperm-cervical mucus contact test(SCMC).The results showed that in fertile men there was no positive sample of spermatozoa before ejaculation in IBT and SCMC.In vasectomized men positive samples were found in 79.4% for IgG,38.2% for IgA and 35.5% for SCMC.The ASABs can be determined in less than one year and remain for more than 3 years after vasectomy.The ASABs were still found on the semen samples during 1-3 months after vasovasostomy.Our results also indicated that the incidence of ASABs on spermatozoa before ejaculation from vasectomized men could not predict the levels of ASABs on their ejaculated sperms after vasovasostomy.There was no correlation between the levels of ASABs in serum before vasovasostomy and those in spermatozoa before ejaculation.