溶脲脲原体感染对大鼠生育及胚胎发育影响的研究

９０只雄性ＳＤ大鼠随机分为三组。Ａ、Ｂ两组每只动物膀胱内接种溶脲脲原体（ＵｒｅａｐｌａｓｍａＵｒｅａｌｙｔｉｃｕｍ，Ｕ．Ｕ．）悬液１ｍｌ（１０５ＣＣＵ／ｍｌ）；Ｂ组接种３个月后，每日口服（灌胃）美满霉素２０～１００ｍｇ／ｋｇ体重，共１４天。Ｃ组为对照。接种５个月后，所有动物进行交配试验。结果表明，接钟后Ａ组动物睾丸组织Ｕ．Ｕ．再分离率为６２．５％，Ｂ组５％，Ｃ组未检出Ｕ．Ｕ．。Ａ组睾丸Ｕ．Ｕ．阳性的动物左右睾丸重量均明显低于Ｂ组和Ｃ组（Ｐ＜０．０２）。Ａ组有１２例动物不育（３０％），Ｂ组有２例动物不育（１０％），Ｃ组全部生育。Ａ组仔鼠（ｎ＝２４０，５．９７±０．６５ｇ）及胚鼠体重（ｎ＝２９３，３．５６±０．９７ｇ）明显低于Ｂ组（分别为ｎ＝１８２，６．９８±０．２６ｇ和ｎ＝１６１，４．４３±０．７２ｇ）和Ｃ组（分别为ｎ＝３２５，６．８９±０．５３ｇ和ｎ＝３４６，４．１７±０．８７ｇ）。Ａ组还发现死胎、畸胎、皮下出血和宫内生长迟缓现象。提示：Ｕ．Ｕ感染可影响大鼠的生育力及胚胎发育，美满霉素可有效抑制Ｕ．Ｕ．生长，并在一定程度上改善生殖状况。

Effects of Ureaplasma Urealyticum(U.U.)Infection on the Fertility and Embryonic Development of the Rats

Ninety of S.D.male rats were divided randomly into three groups.Each urinary bladder of the each animal in group A and B was inoculated with 1 ml broth containing U.U.(105CCU/ml).After three monthes,the rats in group B were daily given orally Minocin(20～100mg/kg wt.)for 14 days.Each rat in group C was injected 1 ml broth of U.U.into urinary bladder for the control.After five monthes,all of the rats were given mating experiment(1 male with 2 females).The results have shown that the positive rates of U.U.in the testes are 62.5% in group A,5 %in group B and 0 in group C respectively.The testes weight of the rats in group A is significantly lower than that in group B and in group C(P<0.02).Infertile rate is 30%(12 cases)in group A and 10%(2 cases)in group B.The weight of the neonatal rats(5.97±0.65g)and the fetal rats(3.56±0.97g)in group A is dramatically lower than that in group B(6.98±0.26g and 4.43±0.72g respectively)and in group C(6.89±0.53g and 4.17±0.87g respectively).In group A very low birth weight(VLBW) neonatal rats,stillbirth,teratism and subcutaneous bleeding were also observed.It is suggested that U.U.infection might be interfering with the fertility and the embryonic development of the rats.Minocin can efficiently inhibit the growth of the U.U.and improve the fertility as well as the embryonic development.