| 利用MTT法检测肝癌化疗药物的敏感性 |
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| 引用本文： | 覃理灵,廖维甲,梅铭惠. 利用MTT法检测肝癌化疗药物的敏感性[J]. 华夏医学, 2007, 20(1): 39-40 |
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| 作者姓名： | 覃理灵 廖维甲 梅铭惠 |
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| 作者单位： | 桂林医学院附属医院肝胆外科研究室,广西,桂林,541001;桂林医学院附属医院肝胆外科研究室,广西,桂林,541001;桂林医学院附属医院肝胆外科研究室,广西,桂林,541001 |
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| 摘 要： | 目的：探讨肝癌对化疗药物的敏感性，筛选个体敏感的化疗药物。方法：采用MTT比色法，对27例原发性肝癌患者癌细胞进行7种抗癌药物敏感性测定。结果：7种化疗药物的敏感率为奥沙利铂（OXA）92．5％，丝裂霉素（MMC）88．88％，顺铂（DDP）81．48％，吉西他滨（GEM）66．66％，表阿霉素（EADM）62．96％，5-氟脲嘧啶（5-FU）51．85％，羟基喜树碱（HCPT）40．74％。结论：MTT法是一种简便、快捷的肿瘤细胞体外药敏试验方法，为肝癌临床个体化治疗提供了实验依据。
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| 关 键 词： | 肝癌细胞 药敏试验MTT比色法 |
| 文章编号： | 1008-2409(2007)01-0039-02 |
| 修稿时间： | 2006-09-29 |
The anti-cancer drug sensitivity of liver cancer with MTT colormetric assay |
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| QIN Li-ling,LIAO Wei-jia,MEI Ming-hui. The anti-cancer drug sensitivity of liver cancer with MTT colormetric assay[J]. Acta Medicinae Sinica, 2007, 20(1): 39-40 |
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| Authors: | QIN Li-ling LIAO Wei-jia MEI Ming-hui |
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| Affiliation: | QIN Li-ling,LIAO Weijia,MEI Ming-hui The Affiliated Hospital of Guilin Medical College,Guilin 541001,China |
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| Abstract: | Objective: To study the anti-cancer drug sensitivity in liver cancer and screen the sensitive drugs for individual.Methods: The chemosensitivity of 27 liver cancer samples to 7 chemotherapeutic agents was determined by using MTT assay.Results: Seven chemotherapeutic agents showed sensitive rate of OXA,MMC,DDP,GEM,EADM,5-FU,HCPT were 92.5%,88.88%,81.48%,66.66%,62.96%,51.85% and 40.74%,respectively.Conclusion: MTT assay is an easy and quick method for drug sensitivity test in vitro and it provides experimental basis for individual chemotherapy. |
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| Keywords: | liver cancer drug sensitivity MTT colormetric assay |
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