| 以hTERT基因反义核酸抑制端粒酶活性增加白血病细胞对CDDP诱导凋亡的敏感性(英文) |
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| 引用本文： | 张洹,何冬梅.以hTERT基因反义核酸抑制端粒酶活性增加白血病细胞对CDDP诱导凋亡的敏感性(英文)[J].中国肿瘤临床(英文版),2004(1). |
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| 作者姓名： | 张洹 何冬梅 |
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| 作者单位： | 广州暨南大学医学院血液病研究所 510632 |
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| 基金项目： | This project was supported by grants from Foundation of Science and Technology of Ouangzhou city(2001-Z-037-01)，Guangdong Province(021195). |
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| 摘 要： | 目的以 hTERT 反义核酸抑制白血病细胞(HL-60和 K562)端粒酶活性,研究 CDDP 诱导凋亡敏感性的变化。方法全硫代反义核酸由上海生物化学研究所合成和纯化;端粒酶活性用试剂合测定(宝灵曼公司产品);用形态学方法和流式细胞仪检测细胞调亡。结果实验结果显示,hTERT 全硫代反义核酸,通过下调 hTERT 基因表达,显著地抑制端粒酶活性;端粒酶活性下降以后,白血病细胞对 CDDP 诱导调亡的敏感性显著升高。结论以 hTERT 基因反义核酸抑制端粒酶活性增加白血病细胞对 CDDP 诱导凋亡的敏感性。
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| 关 键 词： | hTERT(端粒酶逆转录酶) 全硫代反义核酸(AS PS-ODN) 端粒酶 白血病细胞 顺铂(CDDP) |
Inhibition of Telomerase with hTERT Antisense Increases Susceptibility of Leukemic Cells to CDDP-induced Apoptosis |
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| ZHANG Yuan HE Dongmei Institute of Hematology,Medical College of Jinan University,Guangzhou ,China.Inhibition of Telomerase with hTERT Antisense Increases Susceptibility of Leukemic Cells to CDDP-induced Apoptosis[J].Chinese Journal of Clinical Oncology,2004(1). |
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| Authors: | ZHANG Yuan HE Dongmei Institute of Hematology Medical College of Jinan University Guangzhou China |
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| Institution: | ZHANG Yuan HE Dongmei Institute of Hematology,Medical College of Jinan University,Guangzhou 510632,China |
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| Abstract: | Objective:To investigated the effect of inhibition of telomerase with hTERT antisense on leukemic cells(HL-60 and K562)to CDDP-induced apoptosis.Methods:Antisense phosphorothioate oligodeoxynucleotide(AS PS-ODN)was synthesized and purified.Telomerase activity was detected by Telomerase PCR ELASA kit and cell apoptosis was observed by morphological method and determined by flowcytometry.Results:AS PS-ODN could significantly inhibit telomerase activity by down regulat- ing the hTERT expression,and increase the susceptibility of leukemic ceils to CDDP-induced apoptosis. Conclusion:Inhibition of telomerase with hTERT antisense can increases the susceptibility of leukemic cells to CDDP-induced apoptosis. |
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