Clusteron structure of tick-borne encephalitis virus populations |
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| Affiliation: | 1. Department of Zoology, Southern Illinois University Carbondale, 1125 Lincoln Dr. Carbondale, IL, 62901-6501, United States;2. Department of Forestry, Southern Illinois University Carbondale, 1205 Lincoln Dr., Carbondale, IL, 62901, United States;3. Cooperative Wildlife Research Laboratory, Southern Illinois University Carbondale, 1125 Lincoln Dr., Carbondale, IL, 62901, United States;1. Anses, Laboratoire Rage et Faune Sauvage de Nancy, Unité Surveillance et Eco-Epidémiologie des Animaux Sauvages, Technopôle Agricole et Vétérinaire − Bâtiment H, Domaine de Pixérécourt, CS 40009, 54220 Malzéville, France;2. Office National de la Chasse et de la Faune Sauvage, Auffargis, France;3. University Paris Est, Anses, Laboratoire de Santé Animale, Unité Zoonoses Bactériennes, Maisons-Alfort, France;4. Fédération Nationale des Chasseurs, Issy-Les-Moulineaux, France;5. University Paris Est, Anses, Laboratoire de Santé Animale, Unité Epidémiologie, Maisons-Alfort, France;1. School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima 31, Peru;2. Department of Bioengineering, University of Washington, Seattle, WA, USA;3. London School of Hygiene and Tropical Medicine, London, UK;1. Service de néphrologie, CHU Clémenceau, avenue de la Côte-de-Nacre, 14033 Caen cedex, France;2. Unité de tabacologie, CHU Clémenceau, 14033 Caen cedex, France |
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| Abstract: | Tick-borne encephalitis is a natural focal transmissible zooanthroponosis. The causative agent of the disease is a tick-borne encephalitis virus (TBEV) belonging to the genus Flavivirus of the family Flaviviridae and is widespread in Eurasia. Current TBEV classification based on molecular genetic data comprises three phylogenetically separate subtypes: Far Eastern, European and Siberian (TBEV-Sib). Further differentiation of TBEV isn’t developed, making it difficult to investigate the origins, distribution and evolution of the virus. In the present study we determined the nucleotide sequence of the gene E fragment for 282 TBEV-Sib isolates from Ixodes persulcatus ticks or their pools from various natural foci in Russia. Analysis of these sequences and sequences obtained from the GenBank database (more than 600), made it possible to cluster TBEV-Sib strains by identical amino acid sequences of a glycoprotein E fragment. In total, 18 groups were identified (from 3 to 285 strains in the group). It was shown that TBEV strains belonging to the same group are phylogenetically related and have a territorial attachment showing either a local or a corridor type distribution. These groups were named as clusterons showed to be the smallest unit of TBEV classification. The grouping of TBEV strains allows characterization of endemic areas both in quantitative and qualitative composition of the clusterons. The approach could be successfully used to record and monitor the TBEV populations. |
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| Keywords: | Tick-borne encephalitis virus Clusteron Evolution Phylogeography Classification Population |
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