Genetic and antigenic characterization of Indian foot-and-mouth disease virus serotype O isolates collected during the period 2001 to 2012 |
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| Institution: | 1. Foot-and-Mouth Disease Virus Laboratory, Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad 500032, Andhra Pradesh, India;2. Project Directorate on Foot-and-Mouth Disease, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Nainital 263138, Uttarakhand, India;1. Senior Adviser (Medicine & Neuro), Command Hospital (Air Force), Bengaluru, India;2. Classified Specialist (Medicine & Neuro), INHS Asvini, Mumbai, India;3. Senior Adviser (Radiology), INHS Asvini, Mumbai, India;4. Associate Professor, Department of Medicine, Armed Forces Medical College, Pune 411040, India;5. Resident (Medicine), INHS Asvini, Mumbai, India;1. Department of Chemistry, School of Basic Sciences, Indian Institute of Technology Indore, Khandwa Road, Simrol, Indore 453552, India;2. Centre for Biosciences and Biomedical Engineering, School of Basic Sciences, Indian Institute of Technology Indore, Khandwa Road, Simrol, Indore 453552, India;3. Division of Chemistry and Biological Chemistry, Nanyang Technological University, Singapore;1. Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan;2. Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba-shi, Ibaraki, 305-0856, Japan;3. Value Farm Consulting, 1704–3 Nishiooi, Tsukuba, Ibaraki, 300–1260, Japan;1. Novartis Institute for Tropical Diseases, 10 Biopolis Road, #05-01 Chromos Building, Singapore 138670, Singapore;1. Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India;2. Research and Development Laboratory, National Dairy Development Board, c/o Indian Immunologicals Limited, Hyderabad 500032, India;3. Department of Biotechnology, Acharya Nagarjuna University, Guntur 522510, India;4. Peripheral Veterinary Hospital, Madhavaram Milk Colony, TANUVAS, Chennai 600051, India;5. Adviser, National Dairy Development Board, Anand, India |
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| Abstract: | The phylogenetic analysis of VP1 sequences of the 39 type O foot and mouth virus (FMDV) isolates collected from different regions of India during the year of 2001–12 revealed that all isolates belonged to the Middle East – South Asia (ME-SA) topotype. Based on the amount of divergence among the isolates, the viruses were further classified into three distinct lineages namely Ind 2001, PanAsia and PanAsia-2 as well as a minor, unnamed group. Ind 2001 lineage viruses accounted for most of the current type O outbreaks. At the nucleotide level these isolates showed a divergence of 2% to 14% with an average sequence variation of ∼9.9%. The serological spectrum of the current vaccine strain was studied by using bovine vaccinate serum (BVS) raised against O/IND/R2/75. All the current field isolates (n = 24) were homologous (‘r’ value 0.4 to 1.0) to the vaccine strain. Examination of the amino acid sequences for selection pressure revealed the positive selection at amino acid sites 13 and 45. |
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