Complete genetic characterization of human G2P[6] and G3P[6] rotavirus strains |
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| Affiliation: | 1. Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Leading Program, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;3. Department of Medical Microbiology and Public Health Research Laboratory, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal;4. Department of Health Services, Ministry of Health, Government of Nepal, Kathmandu, Nepal;5. Department of Clinical Infection, Microbiology & Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom |
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| Abstract: | During the 2008–2009 rotavirus season, 10 G3P[6] rotavirus strains were isolated for the first time in Belgium, while an outbreak of G2P[6] strains occurred in the USA in 2005–2006. Partial sequencing of the 11 genome segments of the 10 Belgian G3P[6] strains revealed a clonal origin. Two of these strains, and a G2P[6] strain representative of the American outbreak, were selected and sequenced completely to analyze their evolutionary relationships. Genetic analysis revealed that all strains possessed a DS-1-like genotype constellation. The 2 Belgian G3P[6] strains showed >99% sequence identity at the nucleotide level and the American G2P[6] strain was phylogenetically closely related to the Belgian P[6] strains. These data suggest that reassortment(s) involving VP7 occurred recently, and that the prevalence of DS-1-like P[6] rotavirus strains need to be closely monitored because the currently licensed RVA vaccines contain neither the P[6] genotype nor strains with a complete human DS-1 genotype constellation. |
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