Molecular typing and drug susceptibility of Mycobacterium tuberculosis isolates from Chongqing Municipality,China |
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| Institution: | 1. Yongchuan Hospital, Chongqing Medical University, Chongqing, China;2. Beijing Chest Hospital, Capital Medical University, Beijing, China;3. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;1. Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China;2. Department of Clinical Laboratory, Shanghai Public Health Clinical Center, Affiliated to Fudan University, Shanghai, China;3. Jinan Tuberculosis Reference Laboratory, Jinan Tuberculosis Dispensary, Jinan, China;4. Katharine Hsu International Research Center of Human Infectious Diseases, Shandong Provincial Chest Hospital, Jinan, China;1. Respiratory Diseases Department of Nanlou, Chinese People''s Liberation Army General Hospital, Beijing, China;2. National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;3. Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children''s Hospital, Capital Medical University, Beijing, China;4. Liaoning Provincial Center for Disease Control and Prevention, Shenyang, China;1. Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran;2. Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran;3. Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran;4. Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran;5. Division of Pulmonary and Critical Care, University of Miami, Miami, FL, USA;1. Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;2. Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-tuberculosis Association, Tokyo 204-8533, Japan;3. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Ministry of Science and Technology, Pathum Thani 12120, Thailand;4. Department of Pathophysiology and Host Defense, Research Institute of Tuberculosis, Japan Anti-tuberculosis Association, Tokyo 204-8533, Japan |
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| Abstract: | China’s tuberculosis (TB) burden is second only to that of India worldwide. In Chongqing, the largest municipality in southwestern China, although the prevalence of both TB and drug-resistant TB is higher than in other municipalities, the molecular characteristics and drug susceptibility phenotypes are poorly known. In this study, 297 Mycobacterium tuberculosis isolates from Chongqing were genotyped with spacer oligonucleotide typing (spoligotyping) and 28-locus MIRU-VNTR (24-locus MIRU-VNTR scheme and 4 other loci). Spoligotyping results were compared with drug-resistant profiles. Patients who showed clustering by both spoligotyping and 28-locus MIRU-VNTR were interviewed to investigate their detailed contact history. Our data demonstrated that the Beijing genotype was the most prevalent genotype, and ST1 was the most predominant lineage in Chongqing. The Beijing genotype was significantly associated with ethambutol resistance and multidrug-resistant phenotypes. A combination of the 10 most polymorphic loci permitted to achieve higher discriminatory power than 24-VNTR. In addition, a presumed transmission pathway was observed in a cluster of patients with the same MIRU-VNTR profile. The 10-VNTR locus set is suitable for genotyping of Mycobacterium tuberculosis in Chongqing. |
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