Molecular characterization of Cryptosporidium spp. from wild rats and mice from rural communities in the Philippines |
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| Institution: | 1. Department of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, Australia;2. Office of Environment and Heritage, PO Box?1967, Hurstville, NSW 2220, Australia;3. Australian Museum Research Institute, Australian Museum, 6 College Street, Sydney, NSW 2010, Australia;1. School of Veterinary and Life Sciences, Murdoch University, Perth, Australia;2. Australian Water Quality Centre, South Australian Water Corporation, Adelaide, Australia;3. WaterNSW, Sydney, Australia;4. Water Corporation, Perth, Australia;5. Seqwater, Ipswich, Queensland, Australia;6. China-Australia Joint Research and Training Centre for Veterinary Epidemiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;7. University of Queensland, St Lucia, Queensland, Australia |
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| Abstract: | In order to examine the prevalence of Cryptosporidium in wild rodents in the Philippines and understand the role wild rodents play in the transmission of this parasite to humans and livestock, 194 fecal samples from wild rats and mice from Luzon and Mindoro islands were examined. Molecular screening at the 18S and actin gene loci identified an overall prevalence of 25.8% (95%CI: 19.8, 32.5). Sequence and phylogenetic analysis of both loci identified C. parvum, C. muris, C. scrofarum, rat genotypes I-IV and a C. suis-like genotype in the rat-derived isolates and is the first report of C. suis-like and C. scrofarum in rats. Mixed infections were identified in 24% of the Cryptosporidium positive isolates. Rat genotypes II, III and IV showed high intragenotypic variation at the 18S gene locus compared to the actin locus. |
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