Multiple-locus variable number tandem repeat analysis of Neisseria gonorrhoeae isolates in Russia |
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| Affiliation: | 1. Group of Pharmacogenomics, Institute of Chemical Biology and Fundamental Medicine, Lavrentjeva, 8, Novosibirsk 630090, Russia;2. Laboratory of Molecular Genetics of Microorganisms, Research Institute of Physical Chemical Medicine, Malaya Pirogovskaya, 1a, Moscow 119992, Russia;3. Laboratory of Microbiology, Kulakov Research Center of Obstetrics, Gynaecology and Perinatology, Oparina, 4, Moscow 117997, Russia;1. M.V. Lomonosov Moscow State University of Fine Chemical Technologies, Prospect Vernadskogo, 86, 119571, Moscow, Russia;2. N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, 119991, Moscow, Russia;3. A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect, 33, 119071, Moscow, Russia;4. A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninsky Prospect, 29, 119991, Moscow, Russia;5. Institute for Physical-Chemical Medicine, Malaya Pirogovskaya St., 1a, 119828, Moscow, Russia;1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, 119991 Moscow, Russia;2. Research Institute of Physicochemical Medicine, Malaya Pirogovskaya Str. 1a, 119992 Moscow, Russia;3. Department of Higher Plants, Lomonosov Moscow State University, Lenin Hills 1, 119234 Moscow, Russia;1. Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia;2. Moscow Institute of Physics and Technology, Dolgoprudnyi, Russia;3. Department of Urology, Moscow State Medical Stomatological University, Moscow, Russia;1. Research of Age and Age-associated Conditions Department, National Research Centre for Preventive Medicine, Moscow, Russian Federation;2. Laboratory of Bioinformatics, Scientific Research Institute for Physical-Chemical Medicine, Moscow, Russian Federation;3. Russian Clinical Research Center for Gerontology, Moscow, Russian Federation;4. Department of Cardiology and Molecular Genetics, National Research Centre for Preventive Medicine, Moscow, Russian Federation |
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| Abstract: | In the present study, a multiple-locus variable number tandem repeat analysis (MLVA) was used to assess the molecular epidemiology of Neisseria gonorrhoeae clinical isolates originating from different regions of Russia. MLVA, based on seven loci, was performed on 218 isolates that were previously tested for susceptibility to penicillin, tetracycline and ciprofloxacin and for the presence of certain genetic determinants of drug resistance. In total, 83 MLVA types were identified, indicating that MLVA is a highly discriminatory technique with a Hunter–Gaston discriminatory index of 0.963 (95% CI, 0.950–0.977). MLVA type 16 was shown to be the most prevalent type and is undoubtedly associated with a multidrug resistant phenotype. The spread of MLVA type 16 from Moscow to Irkutsk suggests that this type has a highly successful transmission rate. Hierarchical cluster analysis of the MLVA profiles classified 208 isolates (95%) into six large groups (containing more than 10 isolates). Clusters differed in geographical characteristics and susceptibility profiles. MLVA cluster A comprised in total 34 isolates and was unambiguously associated with multidrug resistance. Most isolates in cluster A carried mutations in penA, ponA, rpsJ, mtrR, gyrA, and parC genes. MLVA cluster D was associated with resistance to penicillin and with mutations in ponA and rpsJ genes and the presence of plasmid-borne bla(TEM-1) gene. MLVA clusters B, C and E were associated with susceptibility to ciprofloxacin and had a lack of mutations in ponA, rpsJ, gyrA, and parC genes. We conclude that MLVA will be a useful tool for N. gonorrhoeae epidemiological studies. |
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| Keywords: | Gonorrhoea MLVA MLST NG-MAST VNTR |
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