Whole genomic analyses of asymptomatic human G1P[6], G2P[6] and G3P[6] rotavirus strains reveal intergenogroup reassortment events and genome segments of artiodactyl origin |
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| Affiliation: | 1. Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan;2. Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India;1. Lactic Acid Bacteria and Probiotics Laboratory, Institute of Agrochemistry and Food Technology (IATA-CSIC), Paterna, Spain;2. Department of Microbiology, Medical Faculty, University of Valencia, Valencia, Spain;1. Department of Microbiology and Astrobiology Program, University of Washington, Seattle 98195, USA;2. School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland 4072, Australia;1. Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;2. Key Laboratory of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, 6 Kexueyuan South Road, Beijing 100080, China;3. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China;4. Glycosciences Laboratory, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom;1. Department of Chemistry and CRC Materiali Polimerici (LaMPo), University of Milan, Via Golgi 19, 20133 Milan, Italy;2. Department of Pharmaceutical Sciences, University of ‘Piemonte Orientale, A. Avogadro’, Largo Donegani 2, Novara, Italy |
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| Abstract: | Although P[6] group A rotaviruses (RVA) cause diarrhoea in humans, they have been also associated with endemics of predominantly asymptomatic neonatal infections. Interestingly, strains representing the endemic and asymptomatic P[6] RVAs were found to possess one of the four common human VP7 serotypes (G1–G4), and exhibited little antigenic/genetic differences with the VP4 proteins/VP4 encoding genome segments of P[6] RVAs recovered from diarrhoeic children, raising interest on their complete genetic constellations. In the present study, we report the overall genetic makeup and possible origin of three such asymptomatic human P[6] RVA strains, RVA/Human-tc/VEN/M37/1982/G1P2A[6], RVA/Human-tc/SWE/1076/1983/G2P2A[6] and RVA/Human-tc/AUS/McN13/1980/G3P2A[6]. G1P[6] strain M37 exhibited an unusual genotype constellation (G1-P[6]-R1-C1-M1-A1-N1-T2-E1-H1), not reported previously, and was found to originate from possible intergenogroup reassortment events involving acquisition of a DS-1-like NSP3 encoding genome segment by a human Wa-like RVA strain. On the other hand, G2P[6] strain 1076 exhibited a DS-1-like genotype constellation, and was found to possess several genome segments (those encoding VP1, VP3, VP6 and NSP4) of possible artiodactyl (ruminants) origin on a human RVA genetic backbone. The whole genome of G3P[6] strain McN13 was closely related to that of asymptomatic human Wa-like G3P[6] strain RV3, and both strains shared unique amino acid changes, which might have contributed to their attenuation. Taken together, the present study provided insights into the origin and complex genetic diversity of P[6] RVAs possessing the common human VP7 genotypes. This is the first report on the whole genomic analysis of a G1P[6] RVA strain. |
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