哮喘患儿sIL-2R与细胞免疫功能检测的临床意义

目的探讨儿童支气管哮喘（简称哮喘）发作期淋巴细胞免疫功能和白细胞介素-2（IL-2）、可溶性白介素2受体（sIL-2R）检测的临床意义。方法应用流式细胞术和酶联免疫吸附试验（ELISA）方法对25例支气管哮喘患儿和21例支气管哮喘缓解期患儿以及20例正常儿童外周血T细胞亚群及血清IL-2和sIL-2R水平进行测定。结果支气管哮喘患儿发作期血清sIL-2R水平明显高于缓解期和对照组，缓解期sIL-2R水平仍高于对照组。IL-2水平明显低于缓解期和对照组。外周血总CD3^＋、CD4^＋、CD4^＋／CD8^＋细胞比值均降低，CD8^＋细胞增高。结论动态监测IL-2和sIL-2R水平含量，观察T细胞亚群变化可以判断哮喘发作的程度、指导治疗和评估预后。

The change and clinical significant of cellular immunity and interleukin in children with bronchial asthma

Objective To investigate the clinical significance of relationship between cellular immunity and interleukin （IL-2,sIL-2R） in children with bronchial asthma . Methods T cell subset was assayed by flow cytometry in 25 children with NS in attack phase,21 children with NS in remission phase and 20 normal children. IL-2 and sIL-2R were measured by ELISA in blood serum in all children. Results The level of IL-2 in blood serum is lower in children with attack phase bronchial asthma than those in remission phase and in control. The level of sIL-2R in blood serum is higher in children with attack phase bronchial asthma than those in remission phase and in control. The total CD3^＋ ,CIM^＋ ,CIM ^＋/CD8^＋ decrease, CD8^＋ increase. Conclusion Monitoring IL-2 and sIL-2R , T cell subset ratio change in children with bronchial asthma in attack phase. Cellular immunity,IL-2 and sIL-2R imbalance may play a role in bronchial asthma pathogensis.