首页 | 本学科首页   官方微博 | 高级检索  
     


Strychnine-sensitive glycine receptors in rat caudatoputamen are expressed by cholinergic interneurons
Authors:Darstein M  Landwehrmeyer G B  Kling C  Becker C M  Feuerstein T J
Affiliation:Laboratory for Experimental Neuropsychiatry, University Hospital, Rigshospitalet, Copenhagen, Denmark.
Abstract:Strychnine-sensitive glycine receptors are ligand-gated anion channels widely expressed in spinal cord and brainstem. Recent functional studies demonstrating glycine-induced release of [(3)H]acetylcholine in rat caudatoputamen suggested the existence of excitatory glycine receptors in that region. Since the expression of glycine receptors in the caudatoputamen had not been reported earlier, we studied the glycine receptor-like immunoreactivity in this structure using a monoclonal antibody (mAb4a) recognizing an epitope common to all of the ligand-binding alpha-subunit variants of the glycine receptor. [Becker et al. (1993) Brain Res. 11, 327-333; Nicola et al. (1992) Neurosci. Lett. 138, 173-178]. Immunohistochemistry with mAb4a disclosed a specific staining of sparsely distributed large neurons in rat caudatoputamen, displaying an immunoreactive signal of lower intensity than that observed in motoneurons in spinal cord. Fluorescent dual labelling demonstrated that glycine receptor-like immunoreactivity co-localizes with choline acetyltransferase-like immunoreactivity in rat caudatoputamen. All neurons with glycine receptor-like immunoreactivity in the caudatoputamen studied were immunoreactive with choline acetyltransferase, and represented a subpopulation of cholinergic neurons (approximately 90% of the somata with choline acetyltransferase-like immunoreactivity).These results suggest that strychnine-sensitive glycine receptors are present on cholinergic interneurons in rat caudatoputamen, supporting the hypothesis that glycine receptors inducing striatal release of [(3)H]acetylcholine may be localized to cholinergic neurons.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号