| 高效液相色谱—蒸发光散射法测定糖肾（浓缩）丸中黄芪甲苷含量 |
| |
| 引用本文： | 周越,陈建伟. 高效液相色谱—蒸发光散射法测定糖肾（浓缩）丸中黄芪甲苷含量[J]. 辽宁中医学院学报, 2013, 0(12): 88-89 |
| |
| 作者姓名： | 周越 陈建伟 |
| |
| 作者单位： | [1]南京中医药大学附属连云港市中医院,江苏连云港222000 [2]南京中医药大学,江苏南京210046 |
| |
| 基金项目： | 连云港市卫生局基金资助项目（1314） |
| |
| 摘 要： | 目的：建立糖肾（浓缩）丸中黄芪甲苷含量测定的方法。方法：采用HPLC-ELSD法,十八烷基硅烷键合硅胶为填充剂;乙腈—水（33∶67）为流动相;柱温：25℃;流速：1.0mL/min;检测器为蒸发光散射检测器;蒸发光散射检测器参数：漂移管温度：50℃,气体流速：3.0L/min;检测温度：40℃。结果：黄芪甲苷浓度在20.40~224.40μg/mL范围内线性关系良好,其平均回收率99.27%,RSD为0.79%（n=6）。结论：本方法准确、快速、方便。
|
| 关 键 词： | 糖肾（浓缩）丸 黄芪甲苷 高效液相色谱—蒸发光散射法 含量测定 |
Determination of Astragaloside IV in Tangshen Condensed Pills by HPLC-ELSD |
| |
| ZHOU Yue,CHEN Jianwei. Determination of Astragaloside IV in Tangshen Condensed Pills by HPLC-ELSD[J]. Journal of Liaoning College of Traditional Chinese Medicine, 2013, 0(12): 88-89 |
| |
| Authors: | ZHOU Yue CHEN Jianwei |
| |
| Affiliation: | 2 ( 1.Lianyungang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Lianyungang 222000, Jiangsu, China; 2.Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China ) |
| |
| Abstract: | Objective ： To establish an method for determination of astragaloside IV in Tangshen Condensed Pills. Methods ： The HPLC-ELSD with the Cjs column was used. The mobile phase was acetonitrile-water （33 ： 67） at a flow rate of 1.0 mE/rain and the temperature of column was 25 ℃, the detector was Varian ELSD. The detector drift tube temperature was 50 ℃ and the gas flow rate was 3.0 L/min. The detection temperature was 40℃ . Results ： There was a good linear correlation in the range 20.40-224.40μg/mL for astragaloside IV . The recovery was 99.27% and RSD was 0.79 （n=6） . Conclusion ： The method was accurate,simple and clipping. |
| |
| Keywords: | Tangshen Condensed Pills astragaloside IV HPLC-ELSD content determination |
| 本文献已被 维普 等数据库收录！ |
