| 白花蛇舌草体外诱导人肝癌HEPG2细胞凋亡的初步研究 |
| |
| 引用本文： | 王春涛,袁晓环,王晓东. 白花蛇舌草体外诱导人肝癌HEPG2细胞凋亡的初步研究[J]. 牡丹江医学院学报, 2008, 29(4) |
| |
| 作者姓名： | 王春涛 袁晓环 王晓东 |
| |
| 作者单位： | 牡丹江医学院,黑龙江,牡丹江,157011;牡丹江医学院,黑龙江,牡丹江,157011;牡丹江医学院,黑龙江,牡丹江,157011 |
| |
| 摘 要： | 目的：初步探讨白花蛇舌草对人肝癌HEPG2细胞的增殖抑制作用。方法：制备白花蛇舌草水提取液，体外培养人肝癌HEPG2细胞，MTT法体外细胞毒实验，琼脂糖凝胶电泳方法检测细胞凋亡。结果：白花蛇舌草浓度在10-80mg／mL时，抑制人肝癌HEPG2细胞生长，且随着剂量的增加抑制作用增强，电泳图片显示DNA梯形降解带纹。结论：白花蛇舌草通过诱导细胞凋亡对人肝癌HEPG2细胞具有增殖抑制作用。
|
| 关 键 词： | 白花蛇舌草 HEPG2细胞 细胞凋亡 MTT法 |
THE ELEMENTARY STUDY OF HEDYOTIS DIFFUSA ON INDUCING HEPATOCELLULAR CARCINOMA CELL LINE HEPG2 APOPTOSIS IN VITRO |
| |
| Wang Chuntao et al. THE ELEMENTARY STUDY OF HEDYOTIS DIFFUSA ON INDUCING HEPATOCELLULAR CARCINOMA CELL LINE HEPG2 APOPTOSIS IN VITRO[J]. Journal of Mudanjiang Medical College, 2008, 29(4) |
| |
| Authors: | Wang Chuntao et al |
| |
| Affiliation: | Wang Chuntao et al(Mudanjiang Medical College Heilongjiang Mudanjiang 157011) |
| |
| Abstract: | Objective:To investigate the inhibited effect of Hedyotic diffusa on the cell proliferation of Human Hepatocellular carcinoma cell line HEPG2.Methods:Making water extract of Hedyotic diffusa,and cultivating Hepatocellular carcinoma cell line HEPG2 in vitro,the method of MTT was applied to detect the multiplication inhibitory action,and agarose gel electrophoresis was used to detect apoptosis.Results:The concentration of Hedyotis diffusa in 10-80 mg /mL could inhibit Human Hepatocellular carcinoma cell from ... |
| |
| Keywords: | hedyotis diffusa HEPG2 cell line apoptosis MTT method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
