尼美舒利对胆管癌QBC939细胞增殖的抑制作用

目的研究选择性环氧合酶-2（cyclooxygenase-2，COX-2）抑制剂尼美舒利对人胆管癌细胞系QBC939细胞增殖的抑制作用。方法应用MTT比色法观察尼美舒利对QBC939细胞增殖的影响，细胞凋亡采用透射电镜及流式细胞仪检测，应用免疫组化法检测尼美舒利对QBC939细胞COX-2及增殖细胞核抗原（PCNA）蛋白表达的影响。结果尼美舒利呈时间、剂量依赖性抑制QBC939细胞的增殖，降低QBC939细胞COX-2和PCNA的表达，流式细胞仪检测结果显示，随药物浓度增加，细胞凋亡率显著增加，透射电镜下可见典型的细胞凋亡形态学改变。结论尼美舒利显著抑制QBC939细胞的增殖，诱导其凋亡，且呈时间、剂量依赖性，其机理可能与其下调细胞内COX-2蛋白表达有关。

Inhibition Effects of Nimesulide on Proliferation in Human Cholangiocarcinoma Cell Line QBC939 in Vitro

Objective To investigate the effects of nimesulide, a selective cyclooxygenase-2 (COX-2) inhibitor, on human cholangiocarcinoma QBC939 cell line in vitro. Methods The effects of nimesulide on QBC939 cells were observed with the following techniques: the influence of nimesulide on the proliferation of QBC939 cells was determined by MTT assay; the apoptosis of QBC939 cells was viewed and measured by transmission electron microscopy and flow cytometry, respectively; the expressions of proliferation cell nuclear antigen (PCNA) and COX-2 of cholangiocarcinoma cells were detected by immunocytochemistry. Results Nimesulide inhibited the expressions of PCNA and COX-2 and the proliferation of cholangiocarcinoma QBC939 cells, whose effects intensified as the dose increased and time elongated. Flow cytometry showed that the apoptotic rates of QBC939 cells increased significantly as the dose of nimesulide increased. The typical morphologic features of apoptosis were also observed by transmission electron microscopy. Conclusion Nimesulide significantly inhibits the proliferation of QBC939 cells in vitro by inducting cell apoptosis, which may be associated with the downregulation of COX-2 expression, and it also presents the features of dose and time dependents.