射频热化疗对膀胱癌术后机体细胞免疫功能的影响

目的：探讨膀胱癌术后射频热化疗对机体细胞免疫功能的影响。方法：58例膀胱移行细胞癌术后患者随机分为热化疗组（n=30)和化疗组（n=28)。采用红细胞花环直接法及放免法，分别检测患者术后1周及热化疗或单纯化疗后第2周外周血T淋巴细胞亚群分布、血清白细胞介素2（IL－2）、肿瘤坏死因子（TNF）含量。30例健康体检者作为正常对照组。结果：热化疗组和化疗组患者术后外周血CD3^ 、CD4^ T淋巴细胞数、CD4^ /CD8^ 比值、IL－2含量显著低于正常对照组（P＜0．01）。CD8^ T淋巴细胞数、TNF含量与正常对照组相比差异无统计学意义。化疗后第2周，热化疗组CD3^ 、CD4^ T淋巴细胞数，血清IL－2含量显著升高，与正常对照组相比差异无统计学意义（P＞0．05），与化疗组相比，差异有统计学意义（P＜0．01）。热化疗组TNF含量升高，与对照组相比差异有统计学意义（P＜0．05）。结论：射频热化疗对膀胱癌患者的细胞免疫功能有促进作用。

Influence of the radiofrequency thermo-chemotherapy on cellular immunity for cases after bladder cancer resection

Aim:To investigate the effects of local bladder radiofrequency thermo chemotherapy on cellular immunity for cases after bladder cancer resection.Methods:A total of 58 cases of bladder cancer after operation were randomly assigned to receive thermo chemotherapy(TCT) and chemotherapy (CT) alone.Thirty normal adults were taken as controls.Serum IL 2,TNF content,and T lymphocyte subgroups of peripheral blood were assayed by radioimmunoassay (RIA) and direct erythrocyted rosette test.The parameters above in 3 groups were measured one week after operation and two weeks after TCT or CT.Results:The results showed that IL 2 content, the percentage of CD 3 +,CD 4 + T cells,and CD 4 +/CD 8 + in TCT and CT groups were significantly depressed ( P <0.01).TNF content had no significant difference between 3 groups ( P >0.05).After treatment IL 2 content,the percentages of CD 3 +,CD 4 + T cells, and CD 4 +/CD 8 + were gradually increased in TCT group and they were significantly higher than those of CT group and had no difference with those of controls ( P <0.01).Conclusion:TCT would be an effective approach to improve cellular immunity in patients with bladder cancer.