| Abstract: | To develop an easy-energy-supply agent, medium-chain fatty acids (MCFAs) liposomes were prepared by thin-layer dispersion, freeze-thawing and dynamic high pressure microfluidization (DHPM)-freeze-thawing methods. Results showed that MCFAs nanoliposomes obtained by the novel method (DHPM-freeze-thawing) exhibited a smaller size (72.6?±?4.9?nm), narrower size distribution (PDI?=?0.175?±?0.005), higher zeta potential (?41.27?±?1.16?mV) and entrapment efficiency (45.9?±?6.0%) compared to the other two methods. In the weight-loaded swimming test of the mice, the high-dose group of MCFAs nanoliposomes indicated a significantly longer swimming time (105?±?31?min, p?p?p?≤?0.001), and a higher hepatic glycogen (15.0?±?3.6?mg/g, p?≤?0.001) than those of the control group (53?±?13?min, 1153.6?±?102.5?mg/L, 12.5?±?1.9?mmol/L and 8.8?±?3.3?mg/g, respectively). However, no significant difference was found between the high-dose group and MCFAs group. The results suggested that MCFAs nanoliposomes could be used as a potential easy-energy-supply agent. |
