|
|||||
|
|
| 头状链霉菌中丝裂霉素C抗性基因mcrAB的克隆及其作用研究 | |
| 引用本文： | 王鲁燕,朱春宝,马文哲,陈代杰,许文思. 头状链霉菌中丝裂霉素C抗性基因mcrAB的克隆及其作用研究[J]. 中国抗生素杂志, 2002, 27(12): 748-752 |
| 作者姓名： | 王鲁燕 朱春宝 马文哲 陈代杰 许文思 |
| 作者单位： | 1. 中国药科大学,南京,210009 2. 上海医药工业研究院,上海,200040 3. 上海来益生物药物研究开发中心,上海,201203 |
| 摘 要： | 丝裂霉素 C属于苯醌类抗肿瘤抗生素 ,在生物体内作为前药 ,经酶催化还原后可阻碍 DNA的复制。我们从头状链霉菌中克隆出丝裂霉素 C抗性基因 mcr AB,测序结果与另一丝裂霉素产生菌—淡紫灰链霉菌的 mcr AB基因序列进行了比较 ,结果表明此序列是高度保守的。同时还构建了带有 mcr AB基因的质粒载体 p YG2 0 6 ,将 p YG2 0 6用原生质体转化法导入变青链霉菌 TK6 4 ,并在此菌中表达 ,大大提高了该菌对丝裂霉素 C的抗性。进一步研究表明 :在有氧条件下 ,mcr AB基因表达的蛋白可抑制菌体对丝裂霉素 C的转化作用。 |
| 关 键 词： | 丝裂霉素C 头状链霉菌 抗性基因 |
| 文章编号： | 1001-8689(2002)12-0748-05 |
| 修稿时间： | 2002-04-23 |
Cloning of mcrAB gene from Streptomyces caespitosus and study on the action of mcrAB |
|
| Wang Lu yan ,Zhu Chun bao ,Ma Wen zhe ,Chen Dai jie ,and Xu Wen si. Cloning of mcrAB gene from Streptomyces caespitosus and study on the action of mcrAB[J]. Chinese Journal of Antibiotics, 2002, 27(12): 748-752 | |
| Authors: | Wang Lu yan Zhu Chun bao Ma Wen zhe Chen Dai jie and Xu Wen si |
| Affiliation: | Wang Lu yan 1,Zhu Chun bao 2,Ma Wen zhe 1,Chen Dai jie 3,and Xu Wen si 2 |
| Abstract: | Mitomycin C (MMC) is an antitumor antibiotic containing quinone. MMC is a prodrug and requires an enzymatic reduction in vivo to interfere with DNA replication. The mcrAB gene was cloned from Streptomyces caespitosus , and the nucleotide sequences was compared with that of Streptomyces lavendulae . It revealed that mcrAB gene was highly conservative. Plasmid pYG206 containing mcrAB gene was constructed and introduced into Streptomyces lividans TK64 by protoplast transformation. The transformants appeared high level resistance to MMC. Further research showed that the protein encoded by mcrAB gene could inhibit transformation of MMC in the presence of O 2. |
| Keywords: | Mitomycin C Streptomyces caespitosus Resistance gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |