| 应用噬菌体肽库筛选NMDA受体抗原表位 |
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| 引用本文： | 李桂新,孙长凯,程桂芝,罗建红,李芳. 应用噬菌体肽库筛选NMDA受体抗原表位[J]. 河北医药, 2006, 28(5): 343-344 |
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| 作者姓名： | 李桂新 孙长凯 程桂芝 罗建红 李芳 |
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| 作者单位： | 116023,大连医科大学附属第一医院检验科;大连医科大学脑疾病研究所;大连医科大学免疫学教研室;浙江大学医学院分子中心 |
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| 摘 要： | 目的利用噬菌体展示随机肽库技术,进行NMDAR1抗原表位研究.方法以R1JHL单克隆抗体作为筛选分子,生物淘洗噬菌体展示随机12肽文库.经过4轮的筛淘,ELISA结合实验鉴定阳性克隆,提取阳性克隆噬菌体单链DNA进行序列分析.结果对阳性噬菌体进行递呈肽氨基酸序列分析,获得一阳性克隆序列HYNLSSDRKVRL,与R1JHL单克隆抗体识别序列相比,两者存在一致性序列DRK.结论 DRK可能为NMDA受体抗原表位.
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| 关 键 词： | 噬菌体随机肽库 单克隆抗体 表位 NMDA受体 |
Selection of the minotope of NMDAR1 from phage displayed random peptide library |
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| LI Guixin, SUN Chang- kai , CHENG Guizhi , et ol.. Selection of the minotope of NMDAR1 from phage displayed random peptide library[J]. Hebei Medical Journal, 2006, 28(5): 343-344 |
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| Authors: | LI Guixin SUN Chang- kai CHENG Guizhi et ol. |
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| Affiliation: | The First Affiliated Hospital of Dalian Medical University, Liaoning , Dalian 116023 |
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| Abstract: | Objective To select minotope of NMDAR1 by using phage displayed random peptide library.Methods R1JHLmcAb was used as target to screen the binding peptide from a 12-mer Ph.D.random peptide library.After four rounds of bipanning,the peptide sequence of positive clones was determined and analyzed by DNA sequencing and ELISA assay.Results A positive clone was found (HYNLSSDRKVRL).There was a consentient sequence DRK in NMDAR1.Conclusion DRK in NMDAR1 might be the B cell epitope recognized by R1JHLMcAb. |
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| Keywords: | phage-displayed random peptide library monoclonalantibody epitope NMDA receptor |
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