Umbilical cord tissue-derived mesenchymal stem cells grow best under GMP-compliant culture conditions and maintain their phenotypic and functional properties |
| |
Authors: | Hartmann Isabel Hollweck Trixi Haffner Silvia Krebs Michaela Meiser Bruno Reichart Bruno Eissner Günther |
| |
Affiliation: | Department of Cardiac Surgery, University of Munich, Marchioninistr. 15, 81377 Munich, Germany |
| |
Abstract: | Mesenchymal stem cells (MSCs) are fibroblast-like multipotent stem cells that can differentiate into cell types of mesenchymal origin. Because of their immune properties and differentiation, potential MSCs are discussed for the use in tissue regeneration and tolerance induction in transplant medicine. This cell type can easily be obtained from the umbilical cord tissue (UCMSC) without medical intervention. Standard culture conditions include fetal bovine serum (FBS) which may not be approved for clinical settings. Here, we analyzed the phenotypic and functional properties of UCMSC under xeno-free (XF, containing GMP-certified human serum) and serum-free (SF) culture conditions in comparison with standard UCMSC cultures. Phenotypically, UCMSC showed no differences in the expression of mesenchymal markers or differentiation capacity. Functionally, XF and SF-cultured UCMSC have comparable adipogenic, osteogenic, and endothelial differentiation potential. Interestingly, the UCMSC-mediated suppression of T cell proliferation in an allogeneic mixed lymphocyte reaction (MLR) is more effective in XF and SF media than in standard FBS-containing cultures. Regarding the mechanism of action of MLR suppression, transwell experiments revealed that in neither UCMSC culture a direct cell-cell contact is necessary for inhibiting T cell proliferation, and that the major effector molecule is prostaglandin E2 (PGE2). Taken together, GMP-compliant growth media qualify for long-term cultures of UCMSC which is important for a future clinical study design in regenerative and transplant medicine. |
| |
Keywords: | UCMSC, Umbilical Cord Derived Mesenchymal Stem Cells eUCMSC, Endothelial differentiated UCMSC XF, Xeno-free SF, Serum-free MLR, Mixed lymphocyte reaction GM#1, FBS-containing Growth Medium #1 SP + 2%HS, StemPro MSC SFM medium, supplemented with 2% GMP-compliant human serum MC, MesenCult ACF medium Indo, Indomethacin vWF, von Willebrand factor. |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|