| 组蛋白去乙酰化酶1 siRNA对HeLa细胞生长和凋亡的影响 |
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| 引用本文： | 李娜,于世英.组蛋白去乙酰化酶1 siRNA对HeLa细胞生长和凋亡的影响[J].华中科技大学学报(医学版),2010,39(1). |
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| 作者姓名： | 李娜 于世英 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院肿瘤中心,武汉,430030 |
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| 基金项目： | 湖北省科技攻关计划资助项目 |
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| 摘 要： | 目的 研究组蛋白去乙酰化酶1(histone deacetylases-1,HDAC-1)siRNA对宫颈癌HeLa细胞HDAC-1蛋白表达、细胞生长和凋亡的影响.方法 HDAC-1 siRNA沉默HDAC-1蛋白;Western blot技术检测HDAC-1蛋白;MTT法和克隆形成实验检测细胞生长抑制情况;流式细胞仪检测细胞凋亡.结果 HDAC-1 siRNA转染48 h可明显下调HeLa细胞HDAC-1蛋白表达;下调HDAC-1 表达明显降低HeLa细胞克隆形成率,抑制细胞增殖;下调HDAC-1诱导HeLa细胞凋亡.结论 HDAC-1 siRNA可能通过诱导凋亡抑制HeLa细胞生长.
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| 关 键 词： | 组蛋白去乙酰化酶1 子宫颈癌 细胞凋亡 |
The Effect of Histone Deacetylases-1 siRNA on the Growth and Apoptosis of HeLa Cells |
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| Li Na,Yu Shiying.The Effect of Histone Deacetylases-1 siRNA on the Growth and Apoptosis of HeLa Cells[J].Journal of Huazhong University of Science and Technology(Health Sciences),2010,39(1). |
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| Authors: | Li Na Yu Shiying |
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| Institution: | Li Na,Yu ShiyingCancer Center,Tongji Hospital,Tongji Medical College,HuazhongUniversity of Science , Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the effect of histone deacetylases-1(HDAC-1)siRNA on the HDAC-1 protein expression,cell growth and apoptosis in HeLa cells.Methods The HDAC-1 protein was knocked down by HDAC-1 siRNA.HDAC-1 protein was detected by Western blot.The cell growth inhibition was assesses by MTT and clone forming assay.Apoptosis was measured by flow cytometry.Results Forty-eight h after transfection of HDAC-1 siRNA,HDAC-1 protein expression in HeLa cells was down-regulated obviously.The down-regulation of... |
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| Keywords: | siRNA histone deacetylases-1 siRNA cervical cancer apoptosis |
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