Overexpression of tissue microRNA10b may help predict glioma prognosis |
| |
| Institution: | 1. Shandong Cancer Hospital and Institute, 440 Jiyan, Jinan 250117, Shandong Province, China;2. The Second Hospital of Shandong University, Shandong Province, China;3. Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China;1. University of Illinois, Department of Neurological Surgery, Chicago, IL 60612, USA;2. Emory University School of Medicine, Department of Neurological Surgery, Atlanta, GA 30307, USA;3. University of Miami Miller School of Medicine, Department of Neurological Surgery, Lois Pope Life Center, 1095 NW 14th Terrace (D4-6), Miami, FL 33136, USA;1. Department of Neurological Surgery, University of Arkansas for Medical Sciences, 4301 W. Markham Street, #507, Little Rock, AR 72205, USA;2. New York University School of Medicine, New York, NY, USA;3. Department of Pediatric Neuro-oncology, New York University School of Medicine, New York, NY, USA;4. Department of Neuroradiology, New York University School of Medicine, New York, NY, USA;5. Department of Neurological Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA;6. Department of Neuropathology, New York University School of Medicine, New York, NY, USA;1. Stroke Unit, Level 4, Central Block, Alfred Hospital, Commercial Road, Melbourne, VIC 3004, Australia;2. Faculty of Medicine Nursing and Health Sciences, Monash University, Melbourne, VIC, Australia;3. Department of Radiology, Alfred Hospital, Melbourne, VIC, Australia;1. Department of Neurosurgery, Kurtköy Ersoy Hospital, Istanbul, Turkey;2. Department of Neurosurgery, Batman State Hospital, Batman, Turkey;3. Department of Orthopedic Surgery, Spine Center, University of California San Francisco, 500 Parnassus Avenue, MU320 West San Francisco, CA 94143-0728, USA |
| |
| Abstract: | We investigated the relationship between microRNA-10b (miR-10b) expression and prognosis in human glioma patients. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to characterize the expression patterns of miR-10b in 128 glioma and 20 normal brain tissues. Clinical information – age, sex, Karnofsky Performance Status (KPS) and World Health Organization (WHO) grade – were also collected. The associations between miR-10b expression and the clinicopathological factors and outcome of glioma patients were statistically analyzed. Expression levels of miR-10b in glioma tissue were significantly higher than in normal brain tissue (P < 0.001). High-grade glioma (WHO grade III and IV) had much higher miR-10b expression levels than low-grade tumors (WHO grade I and II). Additionally, the increased miR-10b expression in the glioma tissues was significantly associated with a low KPS (P = 0.03). Kaplan–Meier survival curves and Cox regression analyses showed that overexpression of miR-10b (P = 0.01) and high grade (P = 0.02) were independent factors predicting poor outcome for glioma patients. Furthermore, subgroup analyses showed that the miR-10b expression level was significantly associated with poor overall survival in glioma patients with high grades (P < 0.001). Up-regulation of miR-10b may have value in predicting clinical outcome in glioma patients, particularly for those with high pathological grades. |
| |
| Keywords: | Glioma microRNA-10b miR-10b Prognosis RT-PCR |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
