抗衰益智方Ⅰ对小鼠学习记忆能力以及海马胆碱乙酰转移酶表达的影响

目的：观察抗衰益智方Ⅰ（KYFⅠ）对正常小鼠学习记忆能力和中枢胆碱乙酰转移酶表达的影响。方法:各组小鼠除正常对照组灌服双蒸水外,中药组(分为低、中、高剂量组）灌服相应剂量KYFⅠ，连续灌胃40 d。从第35 d起灌胃结束后进行Morris水迷宫测试，连续5 d。水迷宫测试结束后检测小鼠海马组织胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）的活性，并分别用免疫组化和Western blotting方法观察小鼠海马ChAT阳性神经元的数量变化以及ChAT蛋白的表达。结果:与对照组比较，KYFⅠ高剂量组小鼠水迷宫学习成绩较好；同时，KYFⅠ高剂量组小鼠海马组织内ChAT活性明显增高，AChE活力降低；免疫组化和Western blotting结果显示KYFⅠ高剂量组小鼠海马CA1区ChAT阳性神经元数量增加，ChAT蛋白表达升高。结论:高剂量KYFⅠ可以提高正常小鼠学习记忆能力，其机制可能与KYFⅠ抑制小鼠海马组织AChE活性，提高ChAT活性、升高ChAT蛋白的表达，从而促进脑内乙酰胆碱（ACh）的合成有关。

Effect of Kangshuaiyizhi formula I on learning and memory behavior and expression of ChAT in hippocampus of mice

AIM: To evaluate the role of kangshuaiyizhi formula I (KYF I, a Chinese medicine) on improving the ability of learning and memory in mice and to explore the mechanism related to choline acetyltransferase (ChAT) in hippocampus. METHODS: The mice were divided into 4 groups by gastragavege of distilled water, low-, middle-, and high-doses of KYF I, respectively for 40 successive days. Morris water maze test was used to assess the behavior performance of the mice with different treatment. The activities of AChE and ChAT in hippocampus were measured and the expression of ChAT in hippocampus was observed by immunohistochemistry and Western blotting after treatment. RESULTS: Compared to control group, the mice in high-dose KYF I group showed better performance in Morris water maze test (P<0.05). The activity of ChAT increased (P<0.01) and the activity of AChE decreased in high-dose KYF I treated mice. Moreover, the number of ChAT positive neurons and the expression of ChAT increased significantly in the hippocampus of high-dose KYF I treated animals (P<0.05). CONCLUSION: High-dose KYF I improves the ability of learning and memory in mice. The results suggest that KYF I promotes the level of ACh in brain by inhibiting the activity of AChE, enhancing the activity of ChAT and upregulating the expression of ChAT in hippocampus.