| Let-7a在人宫颈癌HeLa细胞不同周期时相的表达 |
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| 引用本文： | 周珏宇,马文丽,余海浪,肖维威,郑文岭.Let-7a在人宫颈癌HeLa细胞不同周期时相的表达[J].南方医科大学学报,2009,29(3):442. |
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| 作者姓名： | 周珏宇 马文丽 余海浪 肖维威 郑文岭 |
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| 作者单位： | 南方医科大学基因工程研究所,广东,广州,510515;华南基因组研究中心,广东,广州,510800 |
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| 基金项目： | 广东省生物芯片重点实验室基金 |
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| 摘 要： | 目的 探讨微小RNA let-7a在HeLa细胞不同周期时相的差异表达.方法 应用经典的双胸苷阻断法将HeLa细胞同步化于不同的周期时相,应用流式细胞术检测同步化的效率.应用实时荧光定量RT-PCR方法 检测不同周期时相的HeLa细胞中let-7a的表达水平.结果 HeLa细胞G1、S和G2/M期细胞同步化效率分别约为84.81%、83.65%和77.69%;let-7a在不同周期时相均有表达且存在差异,G1和S期表达水平较低,G2/M期表达水平较高.结论 细胞周期对let-7a的表达有较大的影响,这为理解细胞周期调控的具体机制提供了新的线索.
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| 关 键 词： | HeLa细胞 微小RNA 细胞周期 同步化 |
Time course of let-7a expression in the cell cycle of human cervical carcinoma HeLa cells |
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| ZHOU Jue-yu,MA Wen-li,YU Hai-lang,Xiao Wei-wei,ZHENG Wen-ling.Time course of let-7a expression in the cell cycle of human cervical carcinoma HeLa cells[J].Journal of Southern Medical University,2009,29(3):442. |
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| Authors: | ZHOU Jue-yu MA Wen-li YU Hai-lang Xiao Wei-wei ZHENG Wen-ling |
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| Institution: | ZHOU Jue-yu1,MA Wen-li1,YU Hai-lang1,Xiao Wei-wei1,ZHENG Wen-ling2 1Institute of Genetic Engineering,Southern Medical University,Guangzhou 510515,China,2South China Genomics Research Center,Guangzhou 510800 |
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| Abstract: | Objective To investigate the time course of let-7a microRNA expression in the cell cycle of HeLa cells.Methods HeLa cells were synchronized in G1,S and G2/M phases using double-thymidine block,and the cell cycle phases were defined by flow cytometry.Real-time quantitative RT-PCR was used to examine the expression of let-7a in HeLa cells in different cell cycle phases.Results The synchronization rates of G1,S and G2/M phases were 84.81%,83.65% and 77.69%,respectively.Let-7a was constitutively expressed throu... |
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| Keywords: | HeLa cells microRNA cell cycle synchronization |
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