参麦袋泡茶的质量标准研究 |
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作者姓名: | 向云亚 蓝荣 徐晶晶 |
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作者单位: | 厦门医学院/福建省中药精加工与健康产品开发研究室,福建 361023;厦门医学院/福建省中药精加工与健康产品开发研究室,福建 361023;厦门医学院/福建省中药精加工与健康产品开发研究室,福建 361023 |
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基金项目: | 中药精加工与健康产品开发研究;云南农业大学学院科研项目;福建省中青年教师教育科研项目 |
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摘 要: | 目的:建立参麦袋泡茶的质量控制标准。方法:采用薄层色谱法分别对袋泡茶中的西洋参、菊花进行定性鉴别,并用高效液相色谱法对袋泡茶中的人参皂苷Re、人参皂苷Rb1进行含量测定。色谱条件:色谱柱为Kromasil C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水,流速为1.0m L·min-1,柱温为30℃,检测波长为203nm。结果:西洋参、菊花的薄层色谱特征斑点清晰,阴性对照无干扰;人参皂苷Re线性范围为0.800~10.000μg(r=0.9999),平均加样回收率为99.73%,RSD为1.32%;人参皂苷Rb1线性范围为2.216~25.450μg(r=0.9999),平均加样回收率为102.37%,RSD为1.68%。结论:该方法专属性强,重复性好,适用于参麦袋泡茶的质量控制。
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关 键 词: | 袋泡茶 养阴润肺 薄层色谱 高效液相色谱 质量标准 |
Quality Standard for Compound Shenmai Teabag |
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Authors: | XIANG Yun-ya LAN Rong XU Jing-jing |
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Institution: | (Xiamen Medical College/Fujian Provincial Key Laboratory of Traditional Chinese Medicine Finish Machining and Health Products Development,Xiamen 361023,China.) |
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Abstract: | Objective:To establish the quality standard of compound Shenmai teabag and to provide the quality assurance.Method:Panacis Quinquefolii Radix and Chrysanthemi flos were identified by thin layer chromatography.HPLC method was used to determine the content of Ginsenoside Re and Ginsenoside Rb1.The determination was performed on a Kromasil C18 column(4.6 mm×250 mm,5μm),with the mobile phase of acetonitrile-water.The flow rate was 1.0m L·min-1,the column temperature was set at 30℃and test wavelength was 203 nm.Result:The TLC spots were clear and well-separated without negative interference.Ginsenoside Re showed good linearity at 0.800~10.000μg(r=0.9999),and the average recovery was 99.73%with RSD 1.32%.Ginsenoside Rb1 showed good linearity at 2.216~25.450μg(r=0.9999),and the average recovery was 102.37%with RSD 1.68%.Conclusion:The process is practical and stable,which could be used for quality control of compound Shenmai teabag. |
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Keywords: | Teabag Nourishing Yin and Moistening Lung Thin Layer Chromatography(TLC) High Performance Liquid Chromatography(HPLC) Qualification Standard |
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