| 高效液相色谱法测定九节龙中岩白菜素的含量 |
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| 引用本文： | 宋良科,周为民.高效液相色谱法测定九节龙中岩白菜素的含量[J].中药材,2007,30(4):422-423. |
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| 作者姓名： | 宋良科 周为民 |
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| 作者单位： | 西南交通大学药学院,四川峨眉,614202 |
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| 摘 要： | 目的:测定九节龙中岩白菜素的含量。方法:高效液相色谱法,Hypersil C18ODS色谱柱,柱温25℃,波长275 nm,流动相:甲醇-水(20∶80),流速0.600 ml/min;压强1400 Pa。结果:岩白菜素质量在0.098~0.591μg范围与峰面积值的线性关系良好(r=0.9998),平均回收率102.0%,RSD=1.4%(n=6);在该条件下,九节龙岩白菜素含量在0.59%以上。结论:在本文条件下,九节龙中岩白菜素的含量几乎与矮地茶相同。
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| 关 键 词： | 九节龙 岩白菜素 高效液相色谱法 |
| 文章编号： | 1001-4454(2007)04-0422-03 |
| 修稿时间： | 2006-08-28 |
Content Determination of Bergenin in Ardisia pusilla by HPLC |
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| SONG Liang-ke,ZHOU Wei-min.Content Determination of Bergenin in Ardisia pusilla by HPLC[J].Jorunal of Chinese Medicinal Materials,2007,30(4):422-423. |
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| Authors: | SONG Liang-ke ZHOU Wei-min |
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| Institution: | Medicine College of South-west Jiao-tong University, Emei 614202, China |
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| Abstract: | OBJECTIVE: To determine the content of bergenin in Ardisia pusilla. METHODS: High Performance Liquid Chromatography was performed on Hyersil C18ODS. The chromatorgraphic conditions were as follows: methanol-water (20:80) as mobile phase, flow rate being 0.600 ml/min and detecting wavelength at 275 nm. The column temperature was 25 degrees C. RESULTS: A good linearity of bergenin was shown in range of 0. 098-0. 591 microg (r = 0.9998), the average recovery is 102.0%, RSD = 1.4% (n=6). The content of bergenin in Ardisia pusilla is 59% at least. CONCLUSION: The content of bergenin in Ardisia pusilla and A. japonica is identical. |
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| Keywords: | Ardisia pusilla Bergenin HPLC |
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