Elevation of Anti-Cytokeratin 18 Antibody and Circulating Cytokeratin 18: Anti-Cytokeratin 18 Antibody Immune Complexes in Sera of Patients with Idiopathic Pulmonary Fibrosis |
| |
| Authors: | N Dobashi J Fujita M Murota Y Ohtsuki I Yamadori T Yoshinouchi R Ueda S Bandoh T Kamei M Nishioka T Ishida J Takahara |
| |
| Institution: | (1) First Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan, JP;(2) Third Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan, JP;(3) Department of Pathology, Kochi Medical School, Kochi, Japan, JP;(4) Department of Pathology, Okayama University Medical School, Okayama, Japan, JP;(5) Department of Internal Medicine, Nagoya City University, Nagoya, Japan, JP;(6) Department of Internal Medicine, Kagawa Prefectural Central Hospital, Kagawa, Japan, JP |
| |
| Abstract: | In this study, we hypothesize that anti-cytokeratin 18 (CK18) antibody and CK18:anti-CK18 immune complex increase in sera
in patients with idiopathic pulmonary fibrosis (IPF). To prove the existence of anti-CK18 antibodies in patients' sera, bovine
CK18 was stained with patients' sera using a Western blotting. In patients with IPF, anti-CK18 antibodies were clearly demonstrated
in sera by Western blotting. Then, we tried to establish an enzyme-linked immunosorbent assay (ELISA) to quantify anti-CK18
antibodies and CK18:anti-CK18 immune complexes in sera of patients with IPF. Levels of anti-human CK18 antibodies in sera
of patients with IPF (0.81 ± 0.31, mean ± SD) measured by ELISA were significantly high compared with that of normal volunteers
(0.45 ± 0.06, p < 0.01). In addition, levels of CK18:anti-CK18 antibody complexes in patients' sera (0.64 ± 0.35, man ± SD) significantly
increased compared with those of normal subjects (0.40 ± 0.10, p < 0.01). These results suggest that anti-CK18 antibody and its immune complex may have played a role in the process of lung
injury in IPF.
Accepted for publication 30 March 2000 |
| |
| Keywords: | : Autoantibody— Cytokeratin 18— Idiopathic pulmonary fibrosis— Immune complexes |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
