染料木黄酮对ox-LDL诱导人静脉平滑肌细胞MCP-1表达的影响

目的: 研究染料木黄酮(Gen)对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉血管平滑肌细胞(hUVSMC)单核细胞趋化蛋白-1(MCP-1)mRNA和蛋白表达的影响。方法: 以hUVSMC为对象,采用逆转录聚合酶链反应和酶联免疫吸附试验观察不同浓度Gen(1.0×10-5、3.0×10-5、9.0×10-5 mol/L)对ox-LDL诱导的MCP-1 mRNA和蛋白表达的影响。结果:三种浓度的Gen均能明显降低ox-LDL诱导的hUVSMC MCP-1mRNA 和蛋白的表达,且高剂量与生理浓度的17b-雌二醇有相近的作用。结论:染料木黄酮能下调ox-LDL诱导的hUVSMC MCP-1mRNA和蛋白的表达,这可能是染料木黄酮抗动脉粥样硬化的重要机制之一。

EFFECT OF GENISTEIN ON EXPRESSION OF MONOCYTE CHEMOTACTIC PROTEIN-1 INDUCED BY OXIDIZED LIPOPROTEINS IN HUMAN UMBILICAL SMOOTH MUSCLE CELLS

Objective: To study the effect of genistein(Gen) on expression of monocyte chemotactic protein-1 ( MCP-1) mRNA and MCP-1 induced by oxidized low density lipoprotein (ox-LDL) in human umbilical venous smooth mulscle cells (hUVSMC). Methods: Gen at different doses (1.0×10-5、3.0×10-5、9.0×10-5mol/L) were used to observe the effect on expression of MCP-1 mRNA and MCP-1 induced by ox-LDL in hUVSMC cultures and compared with the effect of 17b-estradiol(17b-E). RT-PCR and ELISA were used to measure expression of MCP-1 mRNA and MCP-1 respectively. Results: Gen significantly inhibited the expression of MCP-1 mRNA and MCP-1 in cell culture supernant . There was no significant difference in the inhibitory effect between high concentration of Gen and physiological level of 17b-E . Conclusion: Gen can down-regulate hUVSMC expression of MCP-1 mRNA and MCP-1. It suggests that this may be the important antiatherogenic mechanism of Gen.