柔肝通络汤对脑缺血再灌注损伤大鼠Bcl-2蛋白表达的影响

目的 探讨柔肝通络汤对脑缺血再灌注损伤大鼠Bcl-2蛋白表达的影响.方法 将78只SD大鼠随机分为5组:假手术组、模型组及柔肝通络汤高、中、低剂量组.假手术组及模型组连续1周灌胃蒸馏水.柔肝通络汤各剂量组分别给予高、中、低剂量的柔肝通络汤灌胃,最后一次灌胃30 min后,制成大脑中动脉栓塞模型,保持缺血状态2h后,分别...

Effect of Rougan Tongluo Decoction on the Expression of Bcl-2 Protein in Rats with Cerebral Ischemia Reperfusion Injury

Objective:To explore the effect of Rougan Tongluo Decoction on the expression of Bcl-2 protein in rats with cerebral ischemia reperfusion injury.Methods:A total of 78 SD rats were randomly divided into 5 group:sham operation group,model control group and Rougan Tongluo Decoctiongroups with high,medium and low dose.Distilled water or high,medium and low concentrations of Rougan Tongluo Decoction were given by gavage for 7 d continuously.After 30 min of the last gavage,the middle cerebral artery embolization model was established;after ischemia for 2 h,the brain was taken at 3 time points of reperfusion 3,12 and 24 h,respectively.Before taking the brain,the rats were evaluated for the neurological deficit degrees;HE staining and protein immunohistochemical staining were performed on rat brain tissue after taking the brain.Results:Compared with the sham group,the neurological deficit scores in the model group were increased(P<0.01),the neuronal cell injury in the infarcted side was aggravated,and the expression of Bcl-2 protein was significantly increased(P<0.01).Compared with the model group,the neurological deficit scores in each of Rougan Tongluo Decoction group was decreased(P<0.05),the neuronal cell injury in the infarcted side of rats was significantly reduced,and the expression of Bcl-2 protein was significantly increased(P<0.01).Conclusion:Rougan Tongluo Decoction can effectively improve rat nerve dysfunction,reduce cell injury after ischemia reperfusion,increase the expression of Bcl-2 protein,and play a role in brain protection.