HPV-16 E6 siRNA与hIL-24基因联合诱导人宫颈癌Ca Ski细胞的凋亡

目的：观察HPV-16 E6 siRNA与hIL-24基因体外共转染，联合诱导人宫颈癌CaSki细胞凋亡的效应。方法：HPV-16 E6 siRNA与hIL-24基因的质粒载体分别以单独或联合的方式转染入宫颈癌CaSki细胞，随后利用RT—PCR技术检测细胞中HPV-16E6癌基因的mRNA水平变化；Western blot分析细胞中抑癌蛋白p53水平的变化；流式细胞技术分析细胞凋亡情况。结果：经HPV-16 E6 siRNA和hIL-24转染后细胞后HPVE6癌基因的mRNA水平均下降，其中联合转染组显著下降（P〈0，05）；抑癌蛋白p53水平均增高，其中联合转染组显著增高，细胞凋亡率均升高，其中联合转染组显著升高（P〈0．05）。结论：HPV-16 E6siRNA与hIL-24基因分别转染宫颈癌CaSki细胞后，均能抑制CaSki细胞中HPV-16E6癌基因的表达，使抑癌蛋白p53恢复活性，诱导宫颈癌CaSki细胞凋亡；两者联合别具有协同效应，能显著提高肿瘤细胞凋亡率。

HPV-16 E6 siRNA associated with hIL-24 gene induces apoptosis of human cervical cancer Ca Ski cells

Objective: To evaluate the apoptosis-inducing effect of HPV-16 E6 siRNA associated with hIL-24 gene on human cervical cancer Ca Ski cells.Methods: HPV-16 E6 siRNA and hIL-24 gene eukaryotic expression vector alone or in combination were transfected into human cervical cancer Ca Ski cells.The expression level of HPV-16 E6 mRNA was detected by RT-PCR.The expression of tumor suppressor protein p53 was analyzed by western blot.The apoptosis of Ca Ski cells was analyzed by flow cytometry analysis.Results: The expression level of HPV16 E6 mRNA decreased in Ca Ski cells in all the three groups after transfection with HPV-16 E6 siRNA and hIL-24 gene eukaryotic expression vector.The decrease was significant in co-transfection group(P<0.05).The expression of tumor suppressor protein p53 and the apoptosis rate of Ca Ski cells increased in the three groups and the increase was significant in co-transfection group(P<0.05).Conclusion: Co-transfection of Ca Ski cells with HPV-16 E6 siRNA and hIL-24 gene inhibits the expression of tumor gene HPV E6,recovers the activity of tumor suppressor protein p53,and induces the apoptosis of Ca Ski cells.HPV-16 E6 siRNA associated with hIL-24 gene significantly increases the apoptotic rate of Ca Ski cells.They have synergistic effects.