| 可注射壳聚糖温敏水凝胶对犬骨髓基质细胞生物学行为的影响 |
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| 引用本文： | 韩雪,刘洪臣,王东胜,苏方,鄂玲玲,吴霞.可注射壳聚糖温敏水凝胶对犬骨髓基质细胞生物学行为的影响[J].上海口腔医学,2011,20(2):113-118. |
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| 作者姓名： | 韩雪 刘洪臣 王东胜 苏方 鄂玲玲 吴霞 |
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| 作者单位： | 1. 解放军总医院,口腔医学研究所,北京,100853;沈阳军区总医院,口腔科,辽宁,沈阳,110016
2. 解放军总医院,口腔医学研究所,北京,100853 |
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| 基金项目： | 国家自然科学基金(30973354)~~ |
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| 摘 要： | 目的:探讨可注射壳聚糖(chitosan,CS)基温敏水凝胶对犬骨髓基质细胞(bone marrow stromal cells,BMSCs)生物学活性的影响。方法:利用密度梯度离心法获得犬BMSCs,含10%胎牛血清的L-DMEM培养液进行原代培养并进行初步鉴定。合成壳聚糖温敏水凝胶,光镜下观察BMSCs在凝胶中的存活情况。制备凝胶浸提液,检测其对细胞增殖分化的影响。采用SPSS13.0软件包对实验数据进行单因素方差分析。结果:犬BMSCs可在壳聚糖温敏凝胶中存活。壳聚糖浸提液组对BMSCs的增殖与正常培养液组相比,无显著性差异;但在培养第1、4、7天时,矿化培养液组和矿化浸提液组的细胞增殖率明显低于未矿化的完全培养液组和壳聚糖浸提液组,第10天时,4组间无显著性差异。壳聚糖矿化浸提液组碱性磷酸酶和骨钙素活性明显提高。结论:制备的壳聚糖温敏水凝胶具有良好的生物相容性,可进行水凝胶复合BMSCs的体内外实验研究。
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| 关 键 词： | 壳聚糖 温敏水凝胶 细胞培养 骨髓基质细胞 |
Effects of injectable chitosan thermosensitive hydrogel on dog bone marrow stromal cells in vitro |
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| HAN Xue,LIU Hong-chen,WANG Dong-sheng,SU Fang,E Ling-ling,WU Xia.Effects of injectable chitosan thermosensitive hydrogel on dog bone marrow stromal cells in vitro[J].Shanghai Journal of Stomatology,2011,20(2):113-118. |
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| Authors: | HAN Xue LIU Hong-chen WANG Dong-sheng SU Fang E Ling-ling WU Xia |
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| Institution: | HAN Xue 1,2,LIU Hong-chen 1,WANG Dong-sheng 1,SU Fang 1,E Ling-ling 1,WU Xia 1.(1.Institute of Stomatology,General Hospital of Chinese PLA.Beijing 100853,2.Department of Stomatology,the General Hospital of Shenyang Region.Shenyang 110016,Liaoning Province,China) |
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| Abstract: | PURPOSE: To investigate the biological effects of injectable chitosan(CS) thermosensitive hydrogel on dog bone marrow stromal cells(BMSCs) in vitro.METHODS: Dog BMSCs were obtained from dog bone marrow aspiration by density gradient centrifugation method,cultured in DMEM medium with 10% fetal bovine serum(FBS) and identified.Chitosan thermosensitive hydrogel was prepared and the BMSCs cultured in a medium containing hydrogel were observed under light microscope.The extract liquid from hydrogel was made and ... |
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| Keywords: | Chitosan Thermosensitive hydrogel Cell culture Bone marrow stromal cells |
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