| 小鼠B7-H3对T细胞的体外正性协同刺激作用 |
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| 引用本文： | 孙静,严茹红,顾文超,傅丰庆,张光波,陈旭勤,周迎会,张学光.小鼠B7-H3对T细胞的体外正性协同刺激作用[J].现代免疫学,2011(1):12-16. |
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| 作者姓名： | 孙静 严茹红 顾文超 傅丰庆 张光波 陈旭勤 周迎会 张学光 |
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| 作者单位： | 苏州大学生物技术研究所附属第一人民医院临床免疫学重点实验室;苏州卫生职业技术学院;苏州大学附属第二人民医院; |
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| 基金项目： | 国家自然科学基金资助项目(31040022、30771958); 苏州市社会发展基金资助项目(ss08016) |
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| 摘 要： | 小鼠B7-H3作为协同刺激分子的生物学功能至今尚未明确.本文拟通过构建小鼠B7-H3基因的真核表达载体,获取稳定表达小鼠B7-H3基因的细胞株,并进一步通过体外实验研究其对T淋巴细胞体外活化及功能的调节作用.作者运用RT-PCR技术从小鼠肝脏组织中获得全长小鼠B7-H3基因,并构建真核表达载体pIRES2-B7-H3,...
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| 关 键 词： | 鼠 B7-H3 基因转染 协同刺激作用 T细胞 |
The costimulatory effect of mouse B7-H3 gene in the activation of T cells |
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| SUN Jing,YAN Ru-hong,GU Wen-chao,FU Feng-qing,ZHANG Guang-bo,CHEN Xu-qin,ZHOU Ying-hui,ZHANG Xue-guang.The costimulatory effect of mouse B7-H3 gene in the activation of T cells[J].Current Immunology,2011(1):12-16. |
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| Authors: | SUN Jing YAN Ru-hong GU Wen-chao FU Feng-qing ZHANG Guang-bo CHEN Xu-qin ZHOU Ying-hui ZHANG Xue-guang |
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| Institution: | SUN Jing1,2,YAN Ru-hong1,3,GU Wen-chao1,FU Feng-qing1,ZHANG Guang-bo1,CHEN Xu-qin1,ZHOU Ying-hui1,ZHANG Xue-guang1(1.Institute of Biotechnology,Soochow University,2.Suzhou Health Technology College,3.Clinical Diagnosis Laboratory of the Second Affiliated Hospital,Suzhou 215007,China) |
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| Abstract: | The biological function of mouse B7-H3 was still not identified yet.This paper was aimed to generate an engineered CHO cell line harboring mouse B7-H3 and to investigate its biological function and effects on T lymphocyte activation.The genes coding mouse B7-H3 were obtained from mouse liver cDNA by RT-PCR and then the target gene fragment was inserted into eukaryotic vector pIRES2-EGFP after being digested with EcoR Ⅰ and Bgl Ⅱ to construct the recombinant vector pIRES2-EGFP/B7-H3.This recombinant vector was transfected into CHO cells with LipfectAMINETM 2000 and followed by G418 selection.RT-PCR,Western blotting and flow cytometry(FCM) were used to detect the expression of B7-H3.Effects of the transfected cells on T cells proliferation and cytokine production in vitro was studied by methods of CCK8 and ELISA.In these ways,a stable cell line constitutively expressing the mouse B7-H3 was established successfully,and the mouse B7-H3 could obviously promote the proliferation of T cells in and secretion of IL-2 and IFN-γ in vitro.This effect could be partly blocked by mAb.It is evident that the engineered mouse B7-H3/CHO cell line provides a gain of function cell model for the further understanding of the biological role of B7-H3. |
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| Keywords: | mouse B7-H3 transfected cells co-stimulatory molecules T cells |
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